台湾街头食品耐甲氧西林凝固酶阴性葡萄球菌中 mecA 相关结构。
mecA-related structure in methicillin-resistant coagulase-negative staphylococci from street food in Taiwan.
机构信息
Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan.
Division of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan.
出版信息
Sci Rep. 2017 Feb 9;7:42205. doi: 10.1038/srep42205.
Antibiotic-resistant patterns, a mecA homologue complex, and staphylococcal cassette chromosome mec (SCCmec) were analysed in samples of ready-to-eat (RTE) street food in Taiwan. RTE food samples (270) were collected in three densely populated Taiwanese cities between June and November 2014. Among 14 strains being identified as methicillin-resistant coagulase-negative staphylococci (MRCoNS), genetic diversities was determined by PFGE analysis. SCCmec types IV, V, VIII and TXG-24 were detected in 9, and mecA (a mecA homologue) detected in 8. The mecA gene complex from S. sciuri subsp. sciuri TXG-24 was found to be closely related to those found in both S. sciuri subsp. sciuri (ATCC29062) and S. sciuri subsp. rodentium (ATCC700061). SCCmec carries a class A mec complex, a ccrA5B3-like gene complex, a heavy metal gene complex, and an IS1216 mobile element carrying tet(S). Matching identity to ccrA5 was 84.5% for ccrA in S. pseudintermedius KM241. Matching identify to ccrB3 was 92.1% for ccrB in S. pseudintermedius AI16. Similar ccrA and SCCmec boundary sequences suggest that SCCmec is easily transmitted to coagulase-negative staphylococci (CoNS). Based on MRCoNS strains identified in this research, Taiwanese RTE food products likely carry multiple antibiotic resistance genes that can be transmitted to hospitals and other clinical settings.
在台湾的即食（RTE）街头食品样本中分析了抗生素耐药模式、mecA 同源物复合物和葡萄球菌盒式染色体 mec（SCCmec）。2014 年 6 月至 11 月期间，在台湾三个人口密集的城市中收集了 270 份 RTE 食品样本。在被鉴定为耐甲氧西林凝固酶阴性葡萄球菌（MRCoNS）的 14 株菌株中，通过 PFGE 分析确定了遗传多样性。在 9 株菌中检测到 SCCmec 类型 IV、V、VIII 和 TXG-24，在 8 株菌中检测到 mecA（mecA 同源物）。发现 S. sciuri subsp. sciuri TXG-24 的 mecA 基因复合物与 S. sciuri subsp. sciuri（ATCC29062）和 S. sciuri subsp. rodentium（ATCC700061）中发现的 mecA 基因复合物密切相关。SCCmec 携带 A 类 mec 复合物、ccrA5B3 样基因复合物、重金属基因复合物和携带 tet(S)的 IS1216 移动元件。S. pseudintermedius KM241 中 ccrA 与 ccrA5 的匹配身份为 84.5%。S. pseudintermedius AI16 中 ccrB 与 ccrB3 的匹配身份为 92.1%。相似的 ccrA 和 SCCmec 边界序列表明，SCCmec 很容易传播到凝固酶阴性葡萄球菌（CoNS）。根据本研究中鉴定的 MRCoNS 菌株，台湾的 RTE 食品产品可能携带可传播到医院和其他临床环境的多种抗生素耐药基因。
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