1. Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea; ; 2. Department of Nuclear Medicine, Seoul National University Boramae Hospital, Seoul, Republic of Korea;
1. Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Republic of Korea; ; 3. Institute of Radiation Medicine, Medical Research Center, and.
Theranostics. 2013 Dec 8;3(12):1004-11. doi: 10.7150/thno.4580.
MicroRNAs are small noncoding RNAs regulating gene expression, through base paring with their target mRNAs, which have been actively investigated as key regulators in a wide range of biological processes. Conventional methods such as Northern blot are generally time-consuming, non-repeatable, and cannot be applied in vivo due to the requirement for cell fixation. Therefore, a noninvasive imaging system is required for the monitoring of microRNA biogenesis to understand the versatile functions of microRNAs in vivo as well as in vitro and to accelerate the clinical application of microRNA-based therapy. In this paper, we reviewed noninvasive molecular imaging systems for the monitoring of the biogenesis of microRNAs and post-transcriptional regulation of genes by microRNAs and the roles of microRNAs in various biological processes.
microRNAs 是一类通过与靶 mRNA 的碱基配对来调节基因表达的小非编码 RNA，它们作为关键调控因子在广泛的生物学过程中被广泛研究。传统方法，如 Northern blot，通常耗时、不可重复，并且由于需要细胞固定，因此不能在体内应用。因此，需要一种非侵入性的成像系统来监测 microRNA 的生物发生，以了解 microRNA 在体内以及体外的多功能性，并加速基于 microRNA 的治疗的临床应用。在本文中，我们综述了用于监测 microRNA 生物发生和 microRNA 对基因转录后调控以及 microRNA 在各种生物学过程中的作用的非侵入性分子成像系统。
