Montanari Jorge, Vera María, Mensi Emanuel, Morilla María, Romero Eder
Nanomedicine Research Program, Universidad Nacional de Quilmes, B1876BXD Bernal, Pcia. de Buenos Aires, Argentina.
J Cosmet Sci. 2013 Nov-Dec;64(6):469-81.
With the aim of improving the antioxidant activity of polyphenols from blueberries (Vaccinium myrtillus) on skin targets after topical application, ethanolic extracts from three blueberry varieties (named Millenia, O'Neal, and Blue Crisp) were loaded into ultradeformable liposomes. These nanocarriers are known to be capable of penetrating through the stratum corneum reaching its deeper layers and the viable epidermis. On the other hand, blueberries contain large amounts of polyphenols, whose antioxidant properties as tissue protectors against processes mediated by reactive oxygen species have been extensively proved. Blueberries are usually consumed as edible products, but their antioxidant compounds are poorly absorbed. The antioxidant properties of the extracts were screened before and after being loaded into ultradeformable liposomes made of soy phosphatidylcholine and sodium cholate, of nearly 100 nm in size at 0.223 extract/lipid w/w. The ethanolic extract-loaded ultradeformable liposomes (nanoberries) from Millenia variety retained an 85% of the antioxidant capacity of the free extract and showed low cytotoxicity on HaCaT cells (less than 20%) at active concentration against free radicals.
为了提高蓝莓（欧洲越橘）多酚在局部应用后对皮肤靶点的抗氧化活性，将三个蓝莓品种（名为千年、奥尼尔和蓝脆）的乙醇提取物载入超变形脂质体。已知这些纳米载体能够穿透角质层，到达其更深层和活表皮。另一方面，蓝莓含有大量多酚，其作为组织保护剂对抗由活性氧介导的过程的抗氧化特性已得到广泛证实。蓝莓通常作为可食用产品食用，但其抗氧化化合物的吸收较差。在将提取物载入由大豆磷脂酰胆碱和胆酸钠制成的超变形脂质体之前和之后，对提取物的抗氧化特性进行了筛选，脂质体大小约为100nm，提取物/脂质重量比为0.223。来自千年品种的载乙醇提取物的超变形脂质体（纳米莓）保留了游离提取物85%的抗氧化能力，并且在针对自由基的活性浓度下对HaCaT细胞显示出低细胞毒性（小于20%）。
