Signaling Molecules Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan,
Cell Mol Life Sci. 2014 Jun;71(11):2065-82. doi: 10.1007/s00018-013-1544-5. Epub 2014 Jan 8.
We summarize updated information about DNA microarray-based gene expression profiling by focusing on its application to estrogenic chemicals. First, estrogenic chemicals, including natural/industrial estrogens and phytoestrogens, and the methods for detection and evaluation of estrogenic chemicals were overviewed along with a comprehensive list of estrogenic chemicals of natural or industrial origin. Second, gene expression profiling of chemicals using a focused microarray containing estrogen-responsive genes is summarized. Third, silent estrogens, a new type of estrogenic chemicals characterized by their estrogenic gene expression profiles without growth stimulative or inhibitory effects, have been identified so far exclusively by DNA microarray assay. Lastly, the prospect of a microarray assay is discussed, including issues such as commercialization, future directions of applications and quality control methods.
我们总结了基于 DNA 微阵列的基因表达谱分析的最新信息，重点介绍了其在雌激素化学物质中的应用。首先，概述了包括天然/工业雌激素和植物雌激素在内的雌激素化学物质，以及雌激素化学物质的检测和评估方法，同时列出了天然或工业来源的雌激素化学物质的综合清单。其次，总结了使用包含雌激素反应基因的聚焦微阵列对化学物质进行基因表达谱分析的方法。第三，迄今为止，只有 DNA 微阵列分析才能鉴定出具有雌激素基因表达谱而没有生长刺激或抑制作用的新型雌激素化学物质——沉默雌激素。最后，讨论了微阵列分析的前景，包括商业化、应用的未来方向和质量控制方法等问题。
