Aleksandra A D, Misic M S, Mira Z V, Violeta N M, Dragana I T, Zoran B M, Dejan V S, Milanko S D, Dejan B D
Public Health Institute, Kragujevac; Center for Molecular Medicine and Stem Cell Research, University of Kragujevac, Kragujevac, Serbia.
Indian J Med Microbiol. 2014 Jan-Mar;32(1):49-52. doi: 10.4103/0255-0857.124304.
The emergence of resistance to most antimicrobial agents in staphylococci indicates the need for new effective agents in the treatment of staphylococcal infections. Clindamycin is considered to be one safe, effective and less costly agent. We analysed 482 staphylococcal isolates. Detection of inducible clindamycin resistance was performed by the D-test, while the presence of methylases genes: erm (A), erm (B) and erm (C), as well as, macrolide efflux gene mef was determined by polymerase chain reaction. Inducible clindamycin resistance phenotype was significantly higher in Staphylococcus aureus (S. aureus) strains then in coagulase-negative staphylococci (CNS). Among analysed S. aureus isolates, the predominance of the erm (C) gene, followed by the erm (A) gene were detected. These results indicate that the D-test should be routinely performed on each staphylococcal isolates.
葡萄球菌对大多数抗菌药物产生耐药性，这表明在治疗葡萄球菌感染时需要新的有效药物。克林霉素被认为是一种安全、有效且成本较低的药物。我们分析了482株葡萄球菌分离株。通过D试验检测诱导型克林霉素耐药性，同时通过聚合酶链反应测定甲基化酶基因erm(A)、erm(B)和erm(C)以及大环内酯外排基因mef的存在情况。金黄色葡萄球菌菌株的诱导型克林霉素耐药表型显著高于凝固酶阴性葡萄球菌。在分析的金黄色葡萄球菌分离株中，检测到erm(C)基因占主导地位，其次是erm(A)基因。这些结果表明，应对每株葡萄球菌分离株常规进行D试验。
