Department of Biochemistry, Faculty of Pharmacy, Trakya University , Edirne , Turkey .
Gynecol Endocrinol. 2014 Feb;30(2):165-8. doi: 10.3109/09513590.2013.871516. Epub 2014 Jan 8.
The Gly1057D polymorphism in the insulin receptor substrate-2 (IRS-2) gene has been reported to be associated with insulin resistance, obesity and type 2 diabetes; little is known about its possible association with gestational diabetes mellitus (GDM). To investigate this association we determined the distribution of its genotypes and frequency of alleles in GDM patients.
The study population consisted of 94 subjects; among them were 44 patients with GDM and 50 healthy controls without diabetes. Genomic DNA was extracted from the leukocyte by high pure polymerase chain reaction (PCR) template preparation kit. Genetic polymorphism of IRS-2 G1057D was detected by using PCR-based restriction fragment-length polymorphism (RFLP).
For IRS-2 G1057D polymorphism, there was no significant difference in genotype distribution between GDM patients and controls. The risk for GDM was 2.97 times higher (95% CI: 0.89-9.93, p = 0.076) in the individuals with the IRS-2 DD genotype compared to the GG genotype. Also individuals with the IRS-2 D allele had a significantly higher risk of GDM compared with individuals with the IRS-2 G allele, with a relative risk of 1.86 (95% CI: 1.02-3.37, p = 0.042) for cases compared with population controls.
These results suggest that IRS-2 1057D allele may be associated with GDM.
胰岛素受体底物-2（IRS-2）基因中的 Gly1057D 多态性与胰岛素抵抗、肥胖和 2 型糖尿病有关；但其与妊娠期糖尿病（GDM）的可能相关性知之甚少。为了研究这种相关性，我们确定了其基因型在 GDM 患者中的分布和等位基因的频率。
研究人群包括 94 例受试者；其中包括 44 例 GDM 患者和 50 例无糖尿病的健康对照者。通过高纯度聚合酶链反应（PCR）模板制备试剂盒从白细胞中提取基因组 DNA。采用基于 PCR 的限制性片段长度多态性（RFLP）检测 IRS-2 G1057D 遗传多态性。
对于 IRS-2 G1057D 多态性，GDM 患者和对照组之间的基因型分布无显著差异。与 GG 基因型相比，IRS-2 DD 基因型个体患 GDM 的风险增加了 2.97 倍（95%CI：0.89-9.93，p=0.076）。此外，与 IRS-2 G 等位基因个体相比，IRS-2 D 等位基因个体患 GDM 的风险显著增加，病例与人群对照相比，相对风险为 1.86（95%CI：1.02-3.37，p=0.042）。
这些结果表明 IRS-2 1057D 等位基因可能与 GDM 相关。
