伊朗一些常用于传统医学的药用植物的抗胆碱酯酶活性的初步研究。
A preliminary investigation of anticholinesterase activity of some Iranian medicinal plants commonly used in traditional medicine.
机构信息
Department of Medicinal Chemistry, Faculty of Pharmacy and Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran.
出版信息
Daru. 2014 Jan 8;22(1):17. doi: 10.1186/2008-2231-22-17.
BACKGROUND
The aim of this study was to evaluate acetylcholinesterase inhibitory activity of some commonly used herbal medicine in Iran to introduce a new source for management of Alzheimer's disease. A total of 18 aqueous-methanolic extract (1:1; v/v) from the following plants: Brassica alba, Brassica nigra, Camellia sinensis, Cinchona officinalis, Citrus aurantifolia, Citrus x aurantium, Ferula assafoetida, Humulus lupulus, Juglans regia, Juniperus sabina, Myristica fragrans, Pelargonium graveolens, Pistacia vera, Punica granatum, Rheum officinale, Rosa damascena, Salix alba, and Zizyphus vulgaris were prepared and screened for their acetylcholinesterase inhibitory activity using in vitro Ellman spectrophotometric method.
RESULTS
According to the obtained results, the order of inhibitory activity (IC50 values, μg /ml) of extracts from highest to the lowest was: C. sinensis (5.96), C. aurantifolia (19.57), Z. vulgaris (24.37), B. nigra (84.30) and R. damascena (93.1).
CONCLUSIONS
The results indicated and confirmed the traditional use of these herbs for management of central nervous system disorders. C. sinensis showed the highest activity in inhibition of acetylcholinesterase. However, further investigations on identification of active components in the extracts are needed.
背景
本研究旨在评估一些在伊朗常用草药的乙酰胆碱酯酶抑制活性，以寻找治疗阿尔茨海默病的新来源。共制备了 18 种水-甲醇提取物（1:1；v/v），来自以下植物：白菜、黑甘蓝、茶树、金鸡纳树皮、酸橙、甜橙、阿魏、啤酒花、胡桃、刺柏、肉豆蔻、天竺葵、没药、石榴、大黄、大马士革玫瑰、柳枝和枣。采用体外 Ellman 分光光度法筛选这些提取物对乙酰胆碱酯酶的抑制活性。
结果
根据实验结果，提取物的抑制活性（IC50 值，μg/ml）从高到低的顺序为：茶树（5.96）、酸橙（19.57）、枣（24.37）、黑甘蓝（84.30）和大马士革玫瑰（93.1）。
结论
结果表明并证实了这些草药传统上用于治疗中枢神经系统疾病。茶树对乙酰胆碱酯酶的抑制活性最高。然而，需要进一步研究提取物中活性成分的鉴定。
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