Masini E, Giannella E, Bani-Sacchi T, Fantozzi R, Palmerani B, Mannaioni P F
Agents Actions. 1987 Apr;20(3-4):202-5. doi: 10.1007/BF02074669.
In the present paper we report the results of experiments carried out to measure the release of histamine from isolated rat mast cells during the metabolic activation of arachidonic acid. Arachidonic acid (10(-8)-10(-4) M) and the terminal products (10(-6) M) of the arachidonic acid pathways were devoid of any significant histamine releasing properties. A substantial amount of histamine was released from rat mast cells by low concentrations of arachidonic acid during incubation with prostanoid generating systems, such as guinea-pig lung microsomes, rat serosal macrophages and polymorphonuclear cells and prostaglandin-H-synthase from calf seminal vesicles. The release of histamine was not accompanied by a leakage of lactate dehydrogenase and was blocked by D-mannitol and by lipoxygenase and cyclooxygenase pathway inhibitors. The data are consistent with the hypothesis that free radical derivatives of arachidonic acid, originating from hydroperoxy fatty acids, are generated during catalysis, causing mast cell histamine release.
在本论文中，我们报告了为测量花生四烯酸代谢活化过程中分离的大鼠肥大细胞释放组胺而进行的实验结果。花生四烯酸（10^(-8)-10^(-4) M）以及花生四烯酸途径的终产物（10^(-6) M）均无任何显著的组胺释放特性。在与前列腺素生成系统（如豚鼠肺微粒体、大鼠浆膜巨噬细胞和多形核细胞以及来自小牛精囊的前列腺素-H-合酶）孵育期间，低浓度的花生四烯酸会使大鼠肥大细胞释放大量组胺。组胺的释放并未伴随乳酸脱氢酶的泄漏，并且被D-甘露醇以及脂氧合酶和环氧化酶途径抑制剂所阻断。这些数据与以下假设一致，即花生四烯酸的自由基衍生物源自氢过氧脂肪酸，在催化过程中产生，导致肥大细胞组胺释放。
