Stahl Skov P, Norn S, Weeke B, Nolte H
Agents Actions. 1987 Apr;20(3-4):303-6. doi: 10.1007/BF02074697.
A few patients (6-7%) with a verified type I allergic reaction do not respond with histamine release after challenge of their basophils with specific antigen (non-responding basophils from allergic patients). Sera from these non-responding patients were used for passive sensitization of responding cells from healthy controls. When these sensitized cells were challenged with specific antigen, histamine release was observed indicating that the non-responding allergic patients have circulating antigen-specific IgE capable of binding to Fc-receptors on the basophils. These findings suggest the possibility that non-responding basophils have impaired cell functions. We therefore examined the influence of enhanced IgE receptor stimulation on histamine release in non-responding basophils. This was made by stimulating protein kinase C activity by a phorbol ester (phorbol 12-myristate 13-acetate). When the non-responding cells were incubated with the phorbol ester and challenged with either anti-IgE or specific antigen, the cells released histamine. These findings support the hypothesis that the unresponsiveness of basophils in some allergic patients is associated with impaired IgE receptor complex activation or subcellular functioning and not with a lack of cell-bound IgE.
少数经证实为I型过敏反应的患者（6 - 7%）在用特异性抗原刺激其嗜碱性粒细胞后，不会出现组胺释放反应（来自过敏患者的无反应嗜碱性粒细胞）。来自这些无反应患者的血清用于对健康对照者的反应性细胞进行被动致敏。当用特异性抗原刺激这些致敏细胞时，观察到组胺释放，这表明无反应的过敏患者体内存在能够结合嗜碱性粒细胞上Fc受体的循环抗原特异性IgE。这些发现提示无反应嗜碱性粒细胞可能存在细胞功能受损的可能性。因此，我们研究了增强IgE受体刺激对无反应嗜碱性粒细胞组胺释放的影响。这是通过佛波酯（佛波醇12 - 肉豆蔻酸酯13 - 乙酸酯）刺激蛋白激酶C活性来实现的。当无反应细胞与佛波酯孵育并用抗IgE或特异性抗原刺激时，细胞会释放组胺。这些发现支持了这样一种假说，即某些过敏患者嗜碱性粒细胞的无反应性与IgE受体复合物激活受损或亚细胞功能异常有关，而不是与细胞结合型IgE缺乏有关。
