BioMicroSystems Lab, School of Electronic and Computing Systems, University of Cincinnati, Cincinnati, Ohio 45221, USA.
Biomicrofluidics. 2013 Sep 5;7(5):54101. doi: 10.1063/1.4819275. eCollection 2013.
Blood cell sorting is critical to sample preparation for both clinical diagnosis and therapeutic research. The spiral inertial microfluidic devices can achieve label-free, continuous separation of cell mixtures with high throughput and efficiency. The devices utilize hydrodynamic forces acting on cells within laminar flow, coupled with rotational Dean drag due to curvilinear microchannel geometry. Here, we report on optimized Archimedean spiral devices to achieve cell separation in less than 8 cm of downstream focusing length. These improved devices are small in size (<1 in.(2)), exhibit high separation efficiency (∼95%), and high throughput with rates up to 1 × 10(6) cells per minute. These device concepts offer a path towards possible development of a lab-on-chip for point-of-care blood analysis with high efficiency, low cost, and reduced analysis time.
血细胞分选对于临床诊断和治疗研究的样本制备都至关重要。螺旋惯性微流控装置可以实现无标记、高通量、高效率的细胞混合物连续分离。该装置利用层流中作用于细胞的流体动力，再加上由于曲线微通道几何形状产生的旋转 Dean 曳力。在此，我们报告了优化的阿基米德螺旋装置，以实现在不到 8cm 的下游聚焦长度内进行细胞分离。这些改进后的装置尺寸较小（<1 英寸(2)），分离效率高（约 95%），并且具有高达 1×10(6)个细胞/分钟的高通量。这些器件概念为开发用于即时护理血液分析的片上实验室提供了一种可能，具有高效率、低成本和缩短分析时间的特点。
