Department of Pharmacology, School of Pharmacy, Sungkyunkwan University, Suwon 440-746, Republic of Korea.
Biomol Ther (Seoul). 2013 Nov;21(6):462-9. doi: 10.4062/biomolther.2013.074.
Eucommia ulmoides Oliv. Bark (EUE) is commonly used for the treatment of hypertension, rheumatoid arthritis, lumbago, and ischialgia as well as to promote longevity. In this study, we tested the effects of EUE aqueous extract in graded doses to protect and enhance cognition in scopolamine-induced learning and memory impairments in mice. EUE significantly improved the impairment of short-term or working memory induced by scopolamine in the Y-maze and significantly reversed learning and memory deficits in mice as measured by the passive avoidance and Morris water maze tests. One day after the last trial session of the Morris water maze test (probe trial session), EUE dramatically increased the latency time in the target quadrant in a dose-dependent manner. Furthermore, EUE significantly inhibited acetylcholinesterase (AChE) and thiobarbituric acid reactive substance (TBARS) activities in the hippocampus and frontal cortex in a dose-dependent manner. EUE also markedly increased brain-derived neurotrophic factor (BDNF) and phosphorylation of cAMP element binding protein (CREB) in the hippocampus of scopolamine-induced mice. Based on these findings, we suggest that EUE may be useful for the treatment of cognitive deficits, and that the beneficial effects of EUE are mediated, in part, by cholinergic signaling enhancement and/or protection.
杜仲树皮（EUE）通常用于治疗高血压、类风湿性关节炎、腰痛和坐骨神经痛，以及促进长寿。在这项研究中，我们测试了 EUE 水提物的分级剂量对保护和增强东莨菪碱诱导的学习和记忆损伤的认知能力的作用。EUE 显著改善了东莨菪碱在 Y 迷宫中引起的短期或工作记忆损伤，并通过被动回避和 Morris 水迷宫测试显著逆转了小鼠的学习和记忆缺陷。在 Morris 水迷宫测试的最后一次试验（探针试验）后一天，EUE 以剂量依赖性方式显著增加了目标象限中的潜伏期时间。此外，EUE 还显著抑制了海马体和前额叶皮层中乙酰胆碱酯酶（AChE）和硫代巴比妥酸反应物质（TBARS）的活性，呈剂量依赖性。EUE 还显著增加了东莨菪碱诱导的小鼠海马体中的脑源性神经营养因子（BDNF）和环磷腺苷反应元件结合蛋白（CREB）的磷酸化。基于这些发现，我们认为 EUE 可能对治疗认知障碍有用，并且 EUE 的有益作用部分通过增强胆碱能信号和/或保护来介导。
