Uchiyama Mariko, Yamamoto Kinya, Ochiai Mariko, Yamamoto Tsukasa, Hirano Fumiya, Imamura Saiki, Nagai Hidetaka, Ohishi Kouji, Horiuchi Noriyuki, Kijima Mayumi
National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries, 1-15-1 Tokura, Kokubunji, Tokyo 185-8511, Japan.
Animal Products Safety Division, Food Safety and Consumer Affairs Bureau, Ministry of Agriculture, Forestry and Fisheries, 1-2-1 Kasumigaseki, Chiyoda-ku, Tokyo 100-8950, Japan.
Biologicals. 2014 Mar;42(2):109-13. doi: 10.1016/j.biologicals.2013.12.002. Epub 2014 Jan 7.
Since 2009, erysipelas infection among pigs in Japan has been increasing. This study investigated the prevalence, and characteristics of Erysipelothrix rhusiopathiae isolates in Japan from 2008 to 2010 and assessed the efficacy of current commercial erysipelas vaccines. Based on polymorphisms in a 432-bp hypervariable region in the surface protective antigen A (spaA) gene, 34 isolates were classified into three groups: (i) Group 1 with methionine at position 203 (Met-203) and isoleucine at position 257 (Ile-257) (18 isolates of serotype 1a and one untypable isolate). (ii) Group 2 with Ile-257 (12 isolates of serotypes 1a, 1b, 2, 10 and 11), and (iii) Group 3 with alanine at position 195 (Ala-195) and Ile-257 (three isolates of serotype 1a). Isolates with Met-203 were highly pathogenic in mice and pigs, causing death in the pig and LD50 values of 0.45-1.45 CFU per mouse. One live and three inactivated commercial E. rhusiopathiae vaccines were evaluated for efficacy against a Met-203 isolate. Almost all mice and pigs that received vaccine survived, while non-vaccinated controls all died within 5 days of the challenge. This indicates that swine erysipelas vaccines might be still effective in protecting animals against the recently prevalent Met-203 isolates in Japan.
自2009年以来，日本猪丹毒感染一直在增加。本研究调查了2008年至2010年日本猪丹毒杆菌分离株的流行情况和特征，并评估了当前市售猪丹毒疫苗的效力。基于表面保护性抗原A（spaA）基因中一个432bp高变区的多态性，34株分离株被分为三组：（i）第1组，第203位为甲硫氨酸（Met-203），第257位为异亮氨酸（Ile-257）（18株1a血清型分离株和1株无法分型的分离株）。（ii）第2组，第257位为Ile-257（12株1a、1b、2、10和11血清型分离株），以及（iii）第3组，第195位为丙氨酸（Ala-195）和第257位为Ile-257（3株1a血清型分离株）。具有Met-203的分离株对小鼠和猪具有高度致病性，可导致猪死亡，小鼠的半数致死剂量值为每只小鼠0.45-1.45 CFU。评估了一种活疫苗和三种灭活的市售猪丹毒杆菌疫苗对一株Met-203分离株的效力。几乎所有接种疫苗的小鼠和猪都存活了下来，而未接种疫苗的对照组在攻毒后5天内全部死亡。这表明猪丹毒疫苗可能仍然有效地保护动物免受日本最近流行的Met-203分离株的感染。
