Moriyama H, Fukusaki E, Cabrera Crespo J, Shinmyo A, Okada H
Eur J Biochem. 1987 Aug 3;166(3):539-45. doi: 10.1111/j.1432-1033.1987.tb13547.x.
The complete nucleotide sequence of the beta-xylosidase gene (xynB) of Bacillus pumilus IPO and its flanking regions was established. A 1617-bp open reading frame for beta-xylosidase, a homodimer enzyme, was observed. The amino acid sequence of the N-terminal region and the molecular mass 62607 Da) of the beta-xylosidase subunit, deduced from the DNA sequence, agreed with the result obtained with the purified enzyme. The Shine-Dalgarno sequence was found 8 bp upstream of the initiation codon, ATG. The xylanase gene (xynA) of the same strain was 4.6 kbp downstream of the 3' end of xynB, and its DNA sequence was reported in our previous paper [Fukusaki, E., Panbangred, W., Shinmyo, A. & Okada, H. (1984) FEBS Lett. 171, 197-201]. The results of the Northern hybridization suggested that the mRNA of xynA and xynB were produced separately. The 5' and 3' ends of the xynA and xynB gene were mapped with nuclease S1. The '-10' regions for promoter sequences of both genes were similar to the consensus sequence for B. subtilis RNA polymerases, the '-35' regions were different from all the known promoters for B. subtilis RNA polymerases.
确定了短小芽孢杆菌IPO的β-木糖苷酶基因（xynB）及其侧翼区域的完整核苷酸序列。观察到一个1617bp的β-木糖苷酶开放阅读框，该酶为同型二聚体酶。从DNA序列推导的β-木糖苷酶亚基N端区域的氨基酸序列及其分子量（62607Da）与纯化酶的结果一致。在起始密码子ATG上游8bp处发现了Shine-Dalgarno序列。同一菌株的木聚糖酶基因（xynA）位于xynB 3'端下游4.6kbp处，其DNA序列已在我们之前的论文中报道[Fukusaki, E., Panbangred, W., Shinmyo, A. & Okada, H. (1984) FEBS Lett. 171, 197 - 201]。Northern杂交结果表明xynA和xynB的mRNA是分别产生的。用核酸酶S1对xynA和xynB基因的5'和3'端进行了定位。两个基因启动子序列的“-10”区域与枯草芽孢杆菌RNA聚合酶的共有序列相似，“-35”区域与所有已知的枯草芽孢杆菌RNA聚合酶启动子不同。
