Department of Biology, University of Calgary, T2N 1N4, Calgary, Alta, Canada.
Planta. 1979 Jan;144(4):333-40. doi: 10.1007/BF00391576.
Endo-β-mannanase (EC 3.2.1.78) is produced and secreted by the cells of the endosperm of lettuce (lactuca sativa L.) "seeds" (achenes). In imbibed intact seeds, production is prevented by inhibitors. If the endosperm is incubated alone, these inhibitors can be removed by leaching, allowing mannanase production. Abscisic acid, a component of lettuce seeds, inhibits the production of mannanase in the isolated endosperm, and may be involved in regulation of mannanase production in intact seeds. During germination the inhibition is removed, beginning 4-8 h after red-light irradiation, which was given 4 h from sowing. The cotyledons participate in this process, and are controlled by events occuring in the axis within 4 h from red-light irradiation. This control by the axis apparently depends on the exchange of diffusible substances. Both benzyladenine and gibberellic acid can replace the influence of the axis if the latter is removed, and may therefore be involved in the control by the axis of the rest of the seed.
内切-β-甘露聚糖酶（EC 3.2.1.78）由生菜（莴苣 sativa L.）“种子”（瘦果）的胚乳细胞产生和分泌。在吸胀完整种子中，产物受到抑制剂的阻止。如果单独培养胚乳，这些抑制剂可以通过浸提去除，从而允许甘露聚糖酶的产生。脱落酸是生菜种子的一个成分，它抑制分离胚乳中甘露聚糖酶的产生，并且可能参与调节完整种子中甘露聚糖酶的产生。在萌发过程中，抑制被解除，在播种后 4 小时红光照射开始后 4-8 小时开始。子叶参与这个过程，并且受到红光照射后 4 小时内轴内发生的事件的控制。这种由轴的控制显然取决于可扩散物质的交换。如果去除轴，苄基腺嘌呤和赤霉素都可以取代轴的影响，因此可能参与了轴对种子其余部分的控制。
