儿童哮喘急性期IL-6、IL-17和IL-21水平的变化

The changes in the levels of IL-6, IL-17, and IL-21 in the acute stage of childhood asthma.

作者信息

Tang Xue Qing, Sun Wen Ping, Xu Hong Bo, Liu Wen Bin, Wang Tai Sen, Liu Hua Jun

机构信息

The Department of Pediatrics, the General Hospital of Chengdu Military Region, Chengdu, China 610083.

The Third Department of Neurology, the Affiliated Hospital of Beihua University, Jilin, China, 132013.

出版信息

Clin Lab. 2013;59(11-12):1381-7. doi: 10.7754/clin.lab.2013.121246.

DOI:10.7754/clin.lab.2013.121246

PMID:24409674

Abstract

BACKGROUND

Asthma is the most common disease in children, and its pathogenesis is highly complicated.

METHODS

CD4+ cells and CD4+ IL-17+ cells were analysed by flow cytometry, and the ratio of CD4+ cells to the peripheral blood mononuclear cells (PBMCs) and CD4+ IL-17+ cells to PMBCs were calculated from the control group, acute group, and moderate group. The levels of protein expression of IL-6, IL-17, and IL-21 were detected by ELISA in the plasma and culture supernatants of PBMCs of the three groups. The correlations between IL-7 and IL-6, IL-7 and IL-21 were analysed in culture supernatants of PBMCs of the three groups.

RESULTS

The mean ratio of CD4+ IL-17+ cells/peripheral blood mononuclear cells (PBMCs) was 4.32% in the acute group, which was higher than in the control group and moderate group. The levels of IL-6 and IL-17 were elevated, and the levels of IL-21 were decreased in the acute group. The levels of IL-17 and IL-6 were positively correlated (r = 0.182, p = 0.03), and the levels of IL-17 and IL-21 were negatively correlated (r = -0.834, p = 0.02).

CONCLUSIONS

The results suggest that the levels of IL-17 and IL-6 are related to the severity of asthma. Furthermore, IL-17 may play a role in the development of childhood asthma.

摘要

背景

哮喘是儿童最常见的疾病，其发病机制高度复杂。

方法

采用流式细胞术分析CD4+细胞和CD4+ IL-17+细胞，并计算对照组、急性组和中度组中CD4+细胞与外周血单个核细胞（PBMCs）的比例以及CD4+ IL-17+细胞与PMBCs的比例。采用酶联免疫吸附测定法（ELISA）检测三组PBMCs血浆和培养上清液中IL-6、IL-17和IL-21的蛋白表达水平。分析三组PBMCs培养上清液中IL-7与IL-6、IL-7与IL-21之间的相关性。

结果

急性组CD4+ IL-17+细胞/外周血单个核细胞（PBMCs）的平均比例为4.32%，高于对照组和中度组。急性组IL-6和IL-17水平升高，IL-21水平降低。IL-17与IL-6水平呈正相关（r = 0.182，p = 0.03），IL-17与IL-21水平呈负相关（r = -0.834，p = 0.02）。