Kang Zhen, Zhang Junli, Yang Sen, Du Guocheng, Chen Jian
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China.
School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China.
Sheng Wu Gong Cheng Xue Bao. 2013 Sep;29(9):1214-22.
5-Aminolevulinic acid is the key intermediate of the tetrapyrrole biosynthesis pathway in organisms and has broad application potentials. This review summarized and discussed recent progress in microbial production of 5-aminolevulinic acid, including screening, isolation and mutation of microbes to produce 5-aminolevulinic acid; microbial whole-cell transformation to synthesize 5-aminolevulinic acid depending on the C4 pathway; construction of high-yield 5-aminolevulinic acid producing strains by metabolic engineering. Finally, future research directions in microbial production of 5-aminolevulinic acid were addressed.
5-氨基乙酰丙酸是生物体中四吡咯生物合成途径的关键中间体，具有广阔的应用潜力。本文综述并讨论了微生物生产5-氨基乙酰丙酸的最新进展，包括用于生产5-氨基乙酰丙酸的微生物的筛选、分离和诱变；基于C4途径的微生物全细胞转化合成5-氨基乙酰丙酸；通过代谢工程构建高产5-氨基乙酰丙酸的菌株。最后，探讨了微生物生产5-氨基乙酰丙酸的未来研究方向。
