Tsutsumi H, Flanagan T D, Ogra P L
J Gen Virol. 1987 Aug;68 ( Pt 8):2161-7. doi: 10.1099/0022-1317-68-8-2161.
Employing different routes of immunization and different adjuvants, a large number of hybridomas were produced that secreted monoclonal antibodies (MAbs) against the large envelope glycoprotein (GP90) and the fusion glycoprotein (GP70) of respiratory syncytial virus (RSV). The antigenic specificity of the MAbs was established by immunoblot analysis and radioimmunoprecipitation. Three hybridomas secreting anti-GP90 MAbs were established from mice immunized by intraperitoneal injection of purified RSV in Freund's complete adjuvant. MAbs from these hybridomas did not neutralize viral infectivity. Three other hybridomas established from mice immunized by intraperitoneal inoculation of purified RSV with Bordetella pertussis produced anti-GP90 MAbs that neutralized the virus with or without complement. Similarly three other hybridomas established from mice immunized by infection with RSV via intranasal instillation of the virus produced anti-GP90 MAb that neutralized the virus in the presence and absence of complement. On the other hand, most anti-GP70 MAbs exhibited no neutralizing activity in the absence of complement, although many had low levels of neutralizing activity in the presence of complement, regardless of the type of immunization or the isotype of immunoglobulin. These observations suggest that there may be multiple antigenic sites on GP90, some of which involve a region of the molecule that functions in viral neutralization.
通过采用不同的免疫途径和不同的佐剂，制备了大量杂交瘤，这些杂交瘤分泌针对呼吸道合胞病毒（RSV）大包膜糖蛋白（GP90）和融合糖蛋白（GP70）的单克隆抗体（MAb）。通过免疫印迹分析和放射免疫沉淀确定了单克隆抗体的抗原特异性。从经腹腔注射弗氏完全佐剂中的纯化RSV免疫的小鼠中建立了三个分泌抗GP90单克隆抗体的杂交瘤。这些杂交瘤产生的单克隆抗体不能中和病毒感染性。从经腹腔接种纯化RSV和百日咳博德特氏菌免疫的小鼠中建立的另外三个杂交瘤产生的抗GP90单克隆抗体在有或无补体的情况下均可中和病毒。同样，从经鼻内滴注病毒感染RSV免疫的小鼠中建立的另外三个杂交瘤产生的抗GP90单克隆抗体在有和无补体的情况下均可中和病毒。另一方面，大多数抗GP70单克隆抗体在无补体时无中和活性，尽管许多在有补体时具有低水平的中和活性，无论免疫类型或免疫球蛋白的同种型如何。这些观察结果表明，GP90上可能存在多个抗原位点，其中一些涉及分子中在病毒中和中起作用的区域。
