Tsutsumi H, Onuma M, Suga K, Honjo T, Chiba Y, Chiba S, Ogra P L
Department of Pediatrics, Sapporo Medical College, Japan.
J Clin Microbiol. 1988 Jun;26(6):1171-4. doi: 10.1128/jcm.26.6.1171-1174.1988.
The subgroup characteristics of 71 strains of respiratory syncytial virus (RSV) isolated in Sapporo, Japan, during 5 epidemic years from 1980 to 1987 were determined by the use of 17 monoclonal antibodies (MAbs) raised against the RSV Long strain, which is now recognized as the prototype subgroup A strain. Nine of these MAbs immunoprecipitated the fusion protein (F), five immunoprecipitated the large glycoprotein (G), two immunoprecipitated the nucleoprotein (NP), and one immunoprecipitated the phosphoprotein (P). Based on the pattern of reaction of these MAbs to RSV isolates in an indirect immunofluorescence assay, we were able to distinguish two different subgroups. Subgroup A strains reacted to all 17 MAbs. Subgroup B strains reacted to none of the anti-G MAbs, eight of the nine anti-F MAbs, and all anti-NP and anti-P MAbs. Subgroup A included 38 (53.5%) isolates from every epidemic year. Subgroup B included 32 (45.1%) strains isolated in the last 4 epidemic years. One virus strain with an intermediate character of reactivity was isolated in 1983. From the first epidemic year, six subgroup A strains and no B strains were isolated. During the next three annual epidemics, subgroup B strains were predominantly isolated, i.e., 8 of 13, 10 of 13, and 13 of 17 strains. However, in the last epidemic year only one strain of 22 isolates belonged to subgroup B, and the remainder belonged to subgroup A. This variability of dominance in the occurrence of different RSV subgroup strains may suggest a possible role of the subgroup-specific immune response in RSV epidemics.
1980年至1987年的5个流行年份期间，在日本札幌分离出71株呼吸道合胞病毒（RSV），利用针对RSV Long株产生的17种单克隆抗体（MAb）确定了其亚组特征，RSV Long株现被认为是A亚组原型株。这些MAb中有9种免疫沉淀融合蛋白（F），5种免疫沉淀大糖蛋白（G），2种免疫沉淀核蛋白（NP），1种免疫沉淀磷蛋白（P）。基于这些MAb在间接免疫荧光试验中对RSV分离株的反应模式，我们能够区分出两个不同的亚组。A亚组毒株与所有17种MAb发生反应。B亚组毒株不与任何抗G MAb发生反应，但与9种抗F MAb中的8种、所有抗NP和抗P MAb发生反应。A亚组包括每个流行年份的38株（53.5%）分离株。B亚组包括在最后4个流行年份分离出的32株（45.1%）毒株。1983年分离出1株具有中间反应特征的病毒株。从第一个流行年份开始，分离出6株A亚组毒株，未分离出B亚组毒株。在接下来的三个年度流行中，主要分离出B亚组毒株，即13株中的8株、13株中的10株和17株中的13株。然而，在最后一个流行年份，22株分离株中只有1株属于B亚组，其余属于A亚组。不同RSV亚组毒株出现时优势地位的这种变化可能提示亚组特异性免疫反应在RSV流行中可能发挥的作用。
