淡水鲶鱼（Rita rita）（鲇形目，鲿科）的完整线粒体基因组
Complete mitochondrial genome of the Freshwater Catfish Rita rita (Siluriformes, Bagridae).
作者信息
Lashari Punhal, Laghari Muhammad Younis, Xu Peng, Zhao Zixia, Jiang Li, Narejo Naeem Tariq, Deng Yulin, Sun Xiaowen, Zhang Yan
机构信息
a School of Life Sciences, Beijing Institute of Technology , Beijing , China .
b Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences , Beijing , China , and.
出版信息
Mitochondrial DNA. 2015;26(6):817-8. doi: 10.3109/19401736.2013.855908. Epub 2015 Sep 4.
The complete mitochondrial genome of Catfish, Rita rita, was isolated by LA PCR (TakaRa LAtaq, Dalian, China); and sequenced by Sanger's method to obtain the complete mitochondrial genome, which is listed Critically Endangered and Red Listed species. The complete mitogenome was 16,449 bp in length and contains 13 typical vertebrate protein-coding genes, 2 rRNA and 22 tRNA genes. The whole genome base composition was estimated to be 33.40% A, 27.43% C, 14.26% G and 24.89% T. The complete mitochondrial genome of catfish, Rita rita provides the basis for genetic breeding and conservation studies.
通过长片段PCR（宝生物工程（大连）有限公司的LA Taq酶）分离出了鲇鱼（Rita rita）的完整线粒体基因组；并采用桑格测序法对其进行测序以获得完整的线粒体基因组，该物种被列为极度濒危物种和红色名录物种。完整的线粒体基因组长度为16,449 bp，包含13个典型的脊椎动物蛋白质编码基因、2个rRNA基因和22个tRNA基因。全基因组的碱基组成为：A占33.40%、C占27.43%、G占14.26%、T占24.89%。鲇鱼（Rita rita）的完整线粒体基因组为遗传育种和保护研究提供了依据。
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