Laghari Muhammad Younis, Lashari Punhal, Xu Peng, Zhao Zixia, Jiang Li, Narejo Naeem Tariq, Xin Baoping, Sun Xiaowen, Zhang Yan
a School of Chemical Engineering and Environment, Beijing Institute of Technology , Beijing , China .
b Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences , Beijing , China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):518-9. doi: 10.3109/19401736.2014.905836. Epub 2014 Apr 8.
Complete mitochondrial genome of fresh water giant catfish, Wallago attu, was isolated by LA PCR (TakaRa LAtaq, Dalian, China); and sequenced by Sanger's method to obtain the complete mitochondrial genome. The complete mitogenome was 15,639 bp in length and contains 13 typical vertebrate protein-coding genes, 2 rRNA and 22 tRNA genes. The whole genome base composition was estimated to be 31.17% A, 28.15% C, 15.55% G and 25.12% T. The complete mitochondrial genome of catfish, W. attu, provides the fundamental tools for genetic breeding.
通过长片段PCR（Takara LAtaq，中国大连）分离出淡水巨鲶（Wallago attu）的完整线粒体基因组；并采用桑格法进行测序以获得完整的线粒体基因组。完整的线粒体基因组长度为15,639碱基对，包含13个典型的脊椎动物蛋白质编码基因、2个rRNA基因和22个tRNA基因。整个基因组的碱基组成估计为A占31.17%、C占28.15%、G占15.55%、T占25.12%。鲶鱼W. attu的完整线粒体基因组为遗传育种提供了基础工具。
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