Punhal Lashari, Laghari Muhammad Younis, Waryani Baradi, Jalbani Shaista, Narejo Naeem Tariq, Zhang Yan
a Department of Fresh Water Biology and Fisheries , University of Sindh , Jamshoro , Pakistan and.
b Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences , Beijing , China.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016 Sep;27(5):3224-5. doi: 10.3109/19401736.2015.1007357. Epub 2015 Jan 29.
Complete mitochondrial genome of catfish, Eutropiichthys vacha, was isolated by LA PCR (TakaRa LAtaq, Dalian, China); and sequenced by Sanger's method to obtain the complete mitochondrial genome, which is listed Critically Endangered and Red-listed species. The complete mitogenome was 16,478 bp in length and contains 13 typical vertebrate protein-coding genes, 2 rRNA and 22 tRNA genes. The whole genome base composition was estimated to be 31.06% A, 27.59% C, 15.65% G, and 25.68% T. The complete mitochondrial genome of catfish, E. vacha provides the fundamental tool for genetic breeding and conservation studies.
通过长片段PCR（宝生物工程（大连）有限公司的LA Taq）分离出鲶鱼（Eutropiichthys vacha）的完整线粒体基因组；并采用桑格法进行测序以获得完整的线粒体基因组，该物种被列为极度濒危和红色名录物种。完整的线粒体基因组长度为16,478 bp，包含13个典型的脊椎动物蛋白质编码基因、2个rRNA基因和22个tRNA基因。全基因组碱基组成估计为A占31.06%、C占27.59%、G占15.65%、T占25.68%。鲶鱼E. vacha的完整线粒体基因组为遗传育种和保护研究提供了基础工具。
