Lashari Punhal, Laghari Muhammad Younis, Xu Peng, Zhao Zixia, Jiang Li, Narejo Naeem Tariq, Deng Yulin, Sun Xiaowen, Zhang Yan
a School of Life Sciences, Beijing Institute of Technology , Beijing , China .
b Centre for Applied Aquatic Genomics, Chinese Academy of Fishery Sciences , Beijing , China , and.
Mitochondrial DNA A DNA Mapp Seq Anal. 2016;27(1):439-40. doi: 10.3109/19401736.2014.900612. Epub 2014 Mar 24.
Complete mitochondrial genome of fresh water catfish Bagarius bagarius, was isolated by LA PCR (TakaRa LAtaq, Dalian, China); and sequenced by Sanger's method to obtain the complete mitochondrial genome, which is listed Critically Endangered and Red Listed species. The complete mitogenome was 16,457 bp in length and contains 13 typical vertebrate protein-coding genes, 2 rRNA and 22 tRNA genes. The whole genome base composition was estimated to be 31.14% A, 27.72% C, 15.62% G and 25.50% T. The complete mitochondrial genome of fresh water catfish, B. bagarius provides the basic genetic tools for breeding and conservation program to enhance aquaculture production.
通过长片段PCR（宝生物工程（大连）有限公司的LA Taq酶）分离出淡水鲶鱼黄斑褶鮡的完整线粒体基因组；并采用桑格测序法获得了完整的线粒体基因组，黄斑褶鮡被列为极度濒危和红色名录物种。完整的线粒体基因组长度为16457 bp，包含13个典型的脊椎动物蛋白质编码基因、2个rRNA基因和22个tRNA基因。全基因组碱基组成为：A占31.14%、C占27.72%、G占15.62%、T占25.50%。淡水鲶鱼黄斑褶鮡的完整线粒体基因组为育种和保护计划提供了基础遗传工具，以提高水产养殖产量。
