Chen I-Shiung, Wen Zong-Han, Liao Chiao-Ru, Shen Chia-Ning
a Institute of Marine Biology, National Taiwan Ocean University , Keelung , Taiwan , ROC and.
b Genomic Research Center, Academia Sinica , Taipei , Taiwan , ROC.
Mitochondrial DNA. 2015;26(6):899-901. doi: 10.3109/19401736.2013.861450. Epub 2014 Jan 10.
In this study, the complete mitogenome sequence of balitorid loach Plesiomyzon baotingensis Zheng & Chen, 1980 which was collected from Hainan island, China, has been sequenced by the long polymerase chain reaction method. The mitogenome, consisting of 16,541 base pairs (bp), had typical vertebrate mitochondrial gene arrangement, including 13 protein coding genes, 22 transfer RNAs, 2 ribosomal RNA genes and a noncoding control region (CR). CR of 877 bp length is located between tRNAPro and tRNAPhe. The overall base composition of Plesiomyzon baotingensis is 29.6% for A, 28.5% for C, 25.0% for T and 16.9% for G, with a slight AT bias of 54.6%. The complete mitochondrial genome may provide rather informative data for reconstructing and addressing new molecular perspectives for balitorid phylogenies.
在本研究中，采用长聚合酶链反应方法对采集自中国海南岛的保亭近原吸鳅（Plesiomyzon baotingensis Zheng & Chen, 1980）的完整线粒体基因组序列进行了测序。该线粒体基因组由16,541个碱基对（bp）组成，具有典型的脊椎动物线粒体基因排列，包括13个蛋白质编码基因、22个转运RNA、2个核糖体RNA基因和一个非编码控制区（CR）。长度为877 bp的CR位于tRNAPro和tRNAPhe之间。保亭近原吸鳅的总体碱基组成为：A占29.6%，C占28.5%，T占25.0%，G占16.9%，AT含量略高，为54.6%。完整的线粒体基因组可能为重建近原吸鳅系统发育关系和探讨新的分子视角提供相当丰富的信息数据。
