Chen I-Shiung, Han Meng, Wang Chun-Lin, Shen Chia-Ning
Institute of Marine Biology, National Taiwan Ocean University , Keelung , Taiwan .
Mitochondrial DNA. 2015 Feb;26(1):145-6. doi: 10.3109/19401736.2013.819495. Epub 2013 Jul 31.
In this study, the complete mitogenome sequence of rainbow barbel Acrossocheilus barbodon has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,596 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a noncoding control region (CR). CR of 934 bp length is located between tRNAPro and tRNAPhe. The overall base composition of A. hemispinus is 31.5% for A, 28.2% for C, 24.4% for T and 15.9 % for G, with a higher AC content of 55.9%. The complete mitogenome may provide very essential and important DNA molecular data for further phylogenetic analysis for not only congeneric species but also higher different taxa of Cyprinid fishes.
在本研究中,采用长聚合酶链反应方法扩增并测序了虹彩墨头鱼( Acrossocheilus barbodon )的完整线粒体基因组序列。该线粒体基因组由16,596个碱基对(bp)组成,具有典型的脊椎动物线粒体基因排列,包括13个蛋白质编码基因、22个转运RNA、2个核糖体RNA基因和一个非编码控制区(CR)。长度为934 bp的CR位于tRNAPro和tRNAPhe之间。半刺墨头鱼的总体碱基组成为:A占31.5%,C占28.2%,T占24.4%,G占15.9%,AC含量较高,为55.9%。完整的线粒体基因组不仅可为鲤科鱼类的同属物种,也可为更高分类阶元的进一步系统发育分析提供非常重要的DNA分子数据。