Chen I-Shiung, Wen Zong-Han, Shao Yi-Ta, Liao Chiao-Ru
a Institute of Marine Biology, National Taiwan Ocean University , Keelung , Taiwan , ROC and.
b Institute of Cellular and Organismic Biology, Academia Sinica , Taipei , Taiwan , ROC.
Mitochondrial DNA. 2015;26(6):929-31. doi: 10.3109/19401736.2013.863295. Epub 2014 Jan 10.
In this study, the complete mitogenome sequence of cyprinid fish, Hainan gudgeon Sarcocheilichthys hainanensis Nichols & Pope, 1927 (Cypriniformes: Cyprinidae) has been amplified and sequenced by employing long polymerase chain reaction method. The mitogenome, consisting of 16,674 base pairs (bp), had the typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 22 transfer RNAs, 2 ribosomal RNAs genes and a noncoding control region (CR). CR of 1004 bp length is located between tRNAPro and tRNAPhe. The overall base composition of S. hainanensis is 30.0% for A, 26.7% for C, 26.4% for T and 16.8% for G, with a slight AT bias of 56.4%. The complete mitogenome may provide rather essential and important DNA molecular data for further phylogenetic analysis for not only congeneric species but also higher different taxa of cyprinid fishes.
在本研究中，采用长聚合酶链反应方法扩增并测序了鲤科鱼类海南细鲫（Sarcocheilichthys hainanensis Nichols & Pope，1927）（鲤形目：鲤科）的完整线粒体基因组序列。该线粒体基因组由16674个碱基对（bp）组成，具有典型的脊椎动物线粒体基因排列，包括13个蛋白质编码基因、22个转运RNA、2个核糖体RNA基因和一个非编码控制区（CR）。长度为1004 bp的CR位于tRNAPro和tRNAPhe之间。海南细鲫的总体碱基组成为：A占30.0%，C占26.7%，T占26.4%，G占16.8%，AT偏倚率为56.4%，略呈AT偏倚。完整的线粒体基因组不仅可为鲤科鱼类的同属物种，也可为更高分类阶元的进一步系统发育分析提供相当重要的DNA分子数据。
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