一个与爱泼斯坦-巴尔病毒BamHI M片段转录本对应的cDNA克隆的鉴定：重叠mRNA的证据
Characterization of a cDNA clone corresponding to a transcript from the Epstein-Barr virus BamHI M fragment: evidence for overlapping mRNAs.
作者信息
Pfitzner A J, Strominger J L, Speck S H
出版信息
J Virol. 1987 Sep;61(9):2943-6. doi: 10.1128/JVI.61.9.2943-2946.1987.
A 1.95-kilobase cDNA clone was isolated by screening a size-selected lambda gt10 cDNA library prepared from an Epstein-Barr virus-transformed B-cell line, IB4, with the Epstein-Barr virus BamHI M fragment. Sequence analysis revealed that this clone contains about 75% of the BMRF1 and the complete BMRF2 open reading frames. The transcript is not spliced, and the polyadenylation signal at base pair 2641 of the BamHI M fragment is used. Northern blots (RNA blots) indicate that this polyadenylation signal is used for three overlapping mRNAs. The sizes of these transcripts are 3.5, 2.6, and 1.5 kilobases.
通过用爱泼斯坦 - 巴尔病毒BamHI M片段筛选从爱泼斯坦 - 巴尔病毒转化的B细胞系IB4制备的大小选择的λgt10 cDNA文库，分离出一个1.95千碱基的cDNA克隆。序列分析表明，该克隆包含约75%的BMRF1和完整的BMRF2开放阅读框。该转录本不进行剪接，并使用BamHI M片段第2641碱基对处的聚腺苷酸化信号。Northern印迹（RNA印迹）表明，该聚腺苷酸化信号用于三种重叠的mRNA。这些转录本的大小分别为3.5、2.6和1.5千碱基。
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