编码爱泼斯坦-巴尔病毒核蛋白的信使核糖核酸的核苷酸序列：一个可能的转录起始位点。
Nucleotide sequences of mRNAs encoding Epstein-Barr virus nuclear proteins: a probable transcriptional initiation site.
作者信息
Sample J, Hummel M, Braun D, Birkenbach M, Kieff E
出版信息
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5096-100. doi: 10.1073/pnas.83.14.5096.
Abstract
Three cDNA clones of the second Epstein-Barr virus nuclear antigen (EBNA2) mRNA and two of the EBNA1 mRNA were analyzed. Two EBNA2 clones begin 42 bases 3' to a promoter in the Epstein-Barr virus long internal repeat, which is likely to be the EBNA2 promoter. Surprisingly, the first splice creates an AUG at the beginning of the first of two nonoverlapping open reading frames. The second open reading frame encodes EBNA2. Two incomplete EBNA1 mRNA cDNA clones begin with parts of two of the EBNA2 exons and contain two other exons that map 19 and 59 kilobases 3' to the EBNA2 coding domain. The 3' exon of this mRNA encodes EBNA1. A model for regulation of transcription of these RNAs is presented.
摘要
对第二个爱泼斯坦-巴尔病毒核抗原（EBNA2）mRNA的三个cDNA克隆和EBNA1 mRNA的两个cDNA克隆进行了分析。两个EBNA2克隆在爱泼斯坦-巴尔病毒长内部重复序列中一个启动子的3'端42个碱基处开始，这可能是EBNA2启动子。令人惊讶的是，第一次剪接在两个不重叠的开放阅读框中第一个的开头产生了一个AUG。第二个开放阅读框编码EBNA2。两个不完整的EBNA1 mRNA cDNA克隆以EBNA2的两个外显子的部分开头，并包含另外两个外显子，它们位于EBNA2编码域3'端19和59千碱基处。该mRNA的3'外显子编码EBNA1。提出了这些RNA转录调控的模型。
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