恒河猴口服脂肪耐量试验初步报告。
A preliminary report on oral fat tolerance test in rhesus monkeys.
机构信息
Department of Neurobiology, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University, Beijing 100053, China.
出版信息
Lipids Health Dis. 2014 Jan 10;13:11. doi: 10.1186/1476-511X-13-11.
BACKGROUND
Oral fat tolerance test (OFTT) has been widely used to assess the postprandial lipemia in human beings, but there is few studies concerning OFTT in nonhuman primates. This study is designed to explore the feasibility of OFTT in rhesus monkeys.
METHODS
In a cross-over study, a total of 8 adult female rhesus monkeys were fed with normal monkey diet (NND), high sugar high fat diet (HHD), and extremely high fat diet (EHD), respectively. Each monkey consumed NND, HHD and EHD respectively, each weighing 60 g. Serial blood samples were collected at 1, 2, 3, 4, 5, and 6 h after ingesting each kind of food. Triglyceride, cholesterol, glucose, and insulin at each time point were measured. The area under the curve of triglyceride (TG-AUC) and triglyceride peak response (TG-PR) were also calculated.
RESULTS
All monkeys ingested 3 kinds of foods within 15 minutes. TG-AUC and TG-PR of HHD group were higher than those of the other two groups. Postprandial triglyceride levels at 2, 3, 4, and 5 hours in HHD group during OFTT were also higher than those in NND and EHD group.
CONCLUSIONS
HHD diet can be used in OFTT for nonhuman primates.
背景
口服脂肪耐量试验（OFTT）已广泛用于评估人体的餐后血脂，但关于非人类灵长类动物的 OFTT 研究较少。本研究旨在探讨 OFTT 在恒河猴中的可行性。
方法
在一项交叉研究中，共 8 只成年雌性恒河猴分别喂食正常猴饲料（NND）、高糖高脂饮食（HHD）和极高脂肪饮食（EHD），每种饮食各 60 g。每种食物摄入后 1、2、3、4、5 和 6 h 采集连续血样。在每个时间点测量甘油三酯、胆固醇、葡萄糖和胰岛素。还计算了甘油三酯的曲线下面积（TG-AUC）和甘油三酯峰值反应（TG-PR）。
结果
所有猴子都在 15 分钟内摄入了 3 种食物。HHD 组的 TG-AUC 和 TG-PR 高于其他两组。OFTT 中 HHD 组在 2、3、4 和 5 小时的餐后甘油三酯水平也高于 NND 和 EHD 组。
结论
HHD 饮食可用于非人类灵长类动物的 OFTT。
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