Ruffilli A, Oreste U, Santonastaso V, Scotto D' Abusco A, Sacerdoti G
Mol Immunol. 1987 Mar;24(3):305-12. doi: 10.1016/0161-5890(87)90149-0.
The allergenic composition of a low mol. wt fraction of the pollen extract of Parietaria officinalis (PO) was investigated. Fraction C, that was eluted after oxytocin (mol. wt 1040) when the pollen extract was gel filtered on Sephadex or on Biogel, was cross-reactive in the RAST with the major allergen P015 and was capable of eliciting histamine release from leukocytes of sensitive donors. RAST inhibition (RAST I) analysis of the eluate of gel filtration on Sephadex G-10 revealed several peaks of IgE binding activity. Analysis of fine specificity of response of individual patients carried out by skin-prick tests and by RAST I, revealed individual patterns of reactivity, indicating that allergens contained in fraction C were minor allergens.
对药用墙草（PO）花粉提取物低分子量组分的变应原组成进行了研究。当花粉提取物在葡聚糖凝胶或生物凝胶上进行凝胶过滤时，在催产素（分子量1040）之后洗脱的组分C在放射性变应原吸附试验（RAST）中与主要变应原P015发生交叉反应，并且能够引起敏感供体白细胞释放组胺。对葡聚糖凝胶G-10上凝胶过滤洗脱液的RAST抑制（RAST I）分析显示出几个IgE结合活性峰。通过皮肤点刺试验和RAST I对个体患者反应的精细特异性分析，揭示了个体反应模式，表明组分C中含有的变应原是次要变应原。
