豌豆幼苗可溶性 DNA 聚合酶（聚合酶-α）的部分纯化和性质鉴定

Partial purification and characterization of the soluble DNA polymerase (polymerase-α) from seedlings of Pisum sativum L.

机构信息

Department of Botany, University College, P.O. Box 78, CF1 1XL, Cardiff, UK.

出版信息

Planta. 1978 Jan;138(2):127-32. doi: 10.1007/BF00391168.

Abstract

Soluble DNA polymerase has been extracted from pea seedlings and partially purified by chromatography on columns of DEAE-cellulose or DEAE-Sephadex. The enzyme elutes from DEAE-cellulose as a single peak, but is fractionated into three peaks, SI, SIa and SII by DEAE-Sephadex chromatography. SIa and SII may be derived from SI by freeze-thaw treatment or by treatment with (NH4)2SO4. The ion and pH requirements and the sensitivity to N-ethyl maleimide of the pea seedling soluble DNA polymerase are similar to those of the DNA polymerase-α from vertebrates.

摘要

豌豆幼苗中的可溶 DNA 聚合酶已被提取出来，并通过 DEAE-纤维素或 DEAE-葡聚糖柱层析进行部分纯化。该酶从 DEAE-纤维素上洗脱为单一峰，但通过 DEAE-葡聚糖层析被分成三个峰，即 SI、SIa 和 SII。SIa 和 SII 可能是通过冻融处理或用（NH4）2SO4 处理从 SI 衍生而来的。豌豆幼苗可溶性 DNA 聚合酶的离子和 pH 要求以及对 N-乙基马来酰亚胺的敏感性与脊椎动物的 DNA 聚合酶-α相似。