Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel.
Plant Physiol. 1975 Mar;55(3):437-42. doi: 10.1104/pp.55.3.437.
An 8-fold enhancement in the activity of a DNA-dependent DNA polymerase was found in extracts from germinating wheat (Triticum vulgare var. Florence) embryos, as compared to the activity found in extracts from ungerminated embryos. The enhancement of this activity during the first hours of germination is concomitant to the increase of a Dnase activity. The two activities could be separated and the increased level of the DNA polymerase upon germination was observed in an enzymatic fraction which contains very low DNase activity. Addition of the protein synthesis inhibitor, blasticidin S, to germinating wheat embryos, reduced the increase in DNA polymerase. Incorporation of radioactive amino acids into a phosphocellulose preparation, which contains the DNA polymerase starts during the first 6 hours of germination. The amount of radioactivity incorporated is doubled in the next 6 hours, and the incorporation is continued between 12 and 18 hours of germination.
在萌发的小麦（Triticum vulgare var. Florence）胚胎提取物中发现 DNA 依赖性 DNA 聚合酶的活性比未萌发的胚胎提取物高 8 倍。在萌发的最初几个小时，这种活性的增强伴随着 Dnase 活性的增加。这两种活性可以分离，并且在含有非常低的 Dnase 活性的酶促级分中观察到萌发时 DNA 聚合酶水平的增加。向萌发的小麦胚胎中添加蛋白质合成抑制剂博来霉素 S 可降低 DNA 聚合酶的增加。放射性氨基酸掺入含有 DNA 聚合酶的磷酸纤维素制剂始于萌发的前 6 小时。在下一个 6 小时内掺入的放射性增加了一倍，并且在萌发 12 至 18 小时之间继续掺入。
