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World J Microbiol Biotechnol. 1995 Mar;11(2):144-7. doi: 10.1007/BF00704635.
Restriction analysis of the genomic DNA from a high glucose/xylose-isomerase-yieldingStreptomyces sp. NCIM 2730 revealed a number of distinct bands on a background smear, indicating the occurrence of repeated DNA sequences in the genome. Optical renaturation analysis indicated that 25% of the genome comprised rapidly reannealing sequences with a copy number of 50 and a kinetic complexity of 3×10(3). Hybridization of theStreptomyces genomic library with theStreptomyces DNA, supported the estimate of the repetitive DNA content derived from the re-association kinetics of the DNA. Hybridization of DNA from three differentStreptomyces species with a rice repetitive DNA probe revealed the presence of homologous sequences, which is a unique finding.
对一株高产葡萄糖/木糖异构酶的链霉菌（Streptomyces sp. NCIM 2730）的基因组 DNA 进行限制酶切分析，在背景弥散带中发现了许多明显的条带，表明基因组中存在重复的 DNA 序列。光复性分析表明，25%的基因组由快速复性序列组成，其拷贝数为 50，动力学复杂度为 3×10(3)。用链霉菌 DNA 对链霉菌基因组文库进行杂交，支持了从 DNA 再结合动力学得出的重复 DNA 含量的估计。用水稻重复 DNA 探针与三种不同链霉菌的 DNA 杂交，揭示了同源序列的存在，这是一个独特的发现。
