Zhao X, Kochert G
Department of Botany, University of Georgia, Athens 30602.
Mol Gen Genet. 1992 Feb;231(3):353-9. doi: 10.1007/BF00292702.
A short, highly repeated, interspersed DNA sequence from rice was characterized using a combination of techniques and genetically mapped to rice chromosomes by restriction fragment length polymorphism (RFLP) analysis. A consensus sequence (GGC)n, where n varies from 13-16, for the repeated sequence family was deduced from sequence analysis. Southern blot analysis, restriction mapping of repeat element-containing genomic clones, and DNA sequence analysis indicated that the repeated sequence is interspersed in the rice genome, and is heterogeneous and divergent. About 200,000 copies are present in the rice genome. Single copy sequences flanking the repeat element were used as RFLP markers to map individual repeat elements. Eleven such repeat elements were mapped to seven different chromosomes. The strategy for characterization of highly dispersed repeated DNA and its uses in genetic mapping, DNA fingerprinting, and evolutionary studies are discussed.
利用多种技术对来自水稻的一段短的、高度重复的散布DNA序列进行了表征，并通过限制性片段长度多态性（RFLP）分析将其遗传定位到水稻染色体上。通过序列分析推导出来自该重复序列家族的共有序列（GGC）n，其中n在13至16之间变化。Southern杂交分析、含重复元件的基因组克隆的限制性图谱分析以及DNA序列分析表明，该重复序列散布于水稻基因组中，具有异质性和差异性。水稻基因组中存在约20万个拷贝。位于重复元件侧翼的单拷贝序列被用作RFLP标记来定位各个重复元件。11个这样的重复元件被定位到7条不同的染色体上。本文讨论了高度分散的重复DNA的表征策略及其在遗传图谱构建、DNA指纹识别和进化研究中的应用。
