在酿酒酵母中，铵离子抑制减数分裂：氨对蛋白质代谢的干扰。

Inhibition of meiosis in Saccharomyces cerevisiae by ammonium ions: Interference of ammonia with protein metabolism.

机构信息

Department of Botany, University, Nijmegen, Netherlands.

出版信息

Planta. 1978 Jan;141(2):205-9. doi: 10.1007/BF00387890.

Abstract

Meiosis and sporogenesis in yeast are completely blocked by ammonia added in low concentration (10 mM) to the sporulation medium. Premeiotic DNA synthesis is not initiated in the presence of ammonium ions. The inhibitor interferes with protein turnover by reducing both synthesis and breakdown. The in vitro activities of proteinases A and B in sporulation medium supplemented with ammonia are much lower than in the control. This may partially explain the effect of ammonium ions on protein metabolism in vivo.

摘要

酵母的减数分裂和孢子发生被完全阻断，方法是在孢子形成培养基中添加低浓度（10mM）的氨。在铵离子存在的情况下，不会启动前期 DNA 合成。抑制剂通过减少合成和分解来干扰蛋白质周转。在补充有氨的孢子形成培养基中，蛋白酶 A 和 B 的体外活性明显低于对照。这可能部分解释了铵离子对体内蛋白质代谢的影响。