Esposito M S, Esposito R E, Arnaud M, Halvorson H O
J Bacteriol. 1969 Oct;100(1):180-6. doi: 10.1128/jb.100.1.180-186.1969.
Acetate utilization and macromolecule synthesis during sporulation (meiosis) of Saccharomyces cerevisiae were studied. When diploid cells are transferred from glucose nutrient medium to acetate sporulation medium at early stationary phase, respiration of the exogenously supplied acetate proceeds without any apparent lag. At the completion of ascospore development, 62% of the acetate carbon consumed has been respired, 22% remains in the soluble pool, and 16% is incorporated into lipids, protein, nucleic acids, and other cell components. Measurements of the rate of protein synthesis during sporulation reveal two periods of maximal synthetic activity: an early phase coincidental with increases in deoxyribonucleic acid, ribonucleic acid, and protein cellular content and a later phase during ascospore formation. Experiments in which protein synthesis was inhibited at intervals during sporulation indicate that protein synthesis is required both for the initiation and completion of ascus development.
对酿酒酵母孢子形成（减数分裂）过程中的乙酸利用和大分子合成进行了研究。当二倍体细胞在稳定期早期从葡萄糖营养培养基转移到乙酸孢子形成培养基时，外源供应的乙酸的呼吸作用没有明显延迟地进行。在子囊孢子发育完成时，消耗的乙酸碳中有62%被呼吸，22%留在可溶性池中，16%被整合到脂质、蛋白质、核酸和其他细胞成分中。孢子形成过程中蛋白质合成速率的测量揭示了两个最大合成活性期：一个早期阶段与脱氧核糖核酸、核糖核酸和蛋白质细胞含量的增加同时发生，以及在子囊孢子形成期间的后期阶段。在孢子形成过程中间歇性抑制蛋白质合成的实验表明，蛋白质合成对于子囊发育的起始和完成都是必需的。
