Magee P T, Hopper A K
J Bacteriol. 1974 Sep;119(3):952-60. doi: 10.1128/jb.119.3.952-960.1974.
The dependence upon protein synthesis of physiological and biochemical events occurring during yeast sporulation was investigated. Protein synthesis was inhibited by cycloheximide. There was an early, irreversible sensitivity to inhibition with respect to cell viability and ascus formation; inhibition was reversible only if the cells were inhibited after, but not prior to, 2 to 3 h in sporulation medium. Interruption of protein synthesis of any time during sporulation inhibited all measurable metabolic and sporulation-specific processes except protein breakdown and, to some extent, ribonucleic acid synthesis. The time interval between the occurrence of an event and the protein synthesis necessary for that event was determined to be 2 to 3 h for ascus formation, </=30 min for deoxyribonucleic acid synthesis, 1 h for tetranucleate cell formation, </=30 min for ribonucleic acid breakdown, 1 to 2 h for glycogen synthesis, and 2 to 3 h for glycogen breakdown.
研究了酵母孢子形成过程中发生的生理和生化事件对蛋白质合成的依赖性。蛋白质合成被放线菌酮抑制。在细胞活力和子囊形成方面，对抑制存在早期、不可逆的敏感性；仅当细胞在孢子形成培养基中培养2至3小时之后而非之前受到抑制时，抑制才是可逆的。在孢子形成过程中的任何时间中断蛋白质合成，都会抑制所有可测量的代谢和孢子形成特异性过程，但蛋白质分解以及在一定程度上的核糖核酸合成除外。对于子囊形成，事件发生与该事件所需蛋白质合成之间的时间间隔确定为2至3小时，脱氧核糖核酸合成小于等于30分钟，四核细胞形成1小时，核糖核酸分解小于等于30分钟，糖原合成1至2小时，糖原分解2至3小时。
