嗜热栖热放线菌乙醇耐受性的发展：生长温度的影响
Development of ethanol tolerance in Clostridium thermocellum: effect of growth temperature.
作者信息
Herrero A A, Gomez R F
出版信息
Appl Environ Microbiol. 1980 Sep;40(3):571-7. doi: 10.1128/aem.40.3.571-577.1980.
Abstract
The growth of Clostridium thermocellum ATCC 27405 and of C9, an ethanol-resistant mutant of this strain, at different ethanol concentrations and temperatures was characterized. After ethanol addition, cultures continued to grow for 1 to 2 h at rates similar to those observed before ethanol was added and then entered a period of growth arrest, the duration of which was a function of the age of inocula. After this period, cultures grew at an exponential rate that was a function of ethanol concentration. The wild-type strain showed a higher energy of activation for growth than the ethanol-tolerant derivative. The optimum growth temperature of the wild type decreased as the concentration of the ethanol challenge increased, whereas the optimum growth temperature for C9 remained constant. The results are discussed in terms of what is known about the effects of ethanol and temperature on membrane composition and fluidity.
摘要
对嗜热栖热梭菌ATCC 27405及其乙醇抗性突变体C9在不同乙醇浓度和温度下的生长特性进行了表征。添加乙醇后，培养物以与添加乙醇前观察到的速率相似的速度继续生长1至2小时，然后进入生长停滞期，其持续时间是接种物菌龄的函数。在此期间之后，培养物以指数速率生长，该速率是乙醇浓度的函数。野生型菌株显示出比乙醇耐受衍生物更高的生长活化能。野生型的最佳生长温度随着乙醇挑战浓度的增加而降低，而C9的最佳生长温度保持恒定。根据已知的乙醇和温度对膜组成和流动性的影响对结果进行了讨论。
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