富含微卫星文库的构建。
Development of microsatellite-enriched libraries.
作者信息
Vignes Hélène, Rivallan Ronan
机构信息
Cirad UMR AGAP, Montpellier, France.
出版信息
Methods Mol Biol. 2014;1115:177-89. doi: 10.1007/978-1-62703-767-9_9.
PMID:24415475
Abstract
Among the molecular markers used for plant genetic studies, microsatellite markers are easy to implement and can provide suitable codominant markers for molecular taxonomy. Here we describe a method to obtain enriched libraries in microsatellite loci from genomic DNA, using capture method with synthetic oligonucleotide probes.
摘要
在用于植物遗传学研究的分子标记中，微卫星标记易于操作，可为分子分类学提供合适的共显性标记。本文我们描述了一种从基因组DNA中获得微卫星位点富集文库的方法，该方法使用合成寡核苷酸探针的捕获法。
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