Laboratory Animal and Comparative Medicine Unit, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China.
Gene. 2014 Mar 15;538(1):207-8. doi: 10.1016/j.gene.2014.01.004. Epub 2014 Jan 11.
A standardised methodology has been used to define genotypes based on pairwise sequence comparisons (PASC). PASC is a widely accepted method in virus taxonomy, which is based on the histogram of pairwised differences among sequences. Recently, Zhang et al. (2013) concluded that the average p-distance of duck circovirus (DuCV) between genotypes 1 and 2 was 0.170, and subtype distance thresholds were 0.032 in DuCV-1 and 0.018 in DuCV-2, respectively. However, there might be some concerns on the methodology application to define the genotype of DuCV. Taking into account the concerns mentioned above, our authors conducted the PASC analyses of 54 capsid gene (ORF2) and genomic sequences including all the sequences from Zhang et al. (2013). Our results confirmed the existence of two DuCV genotypes (1 and 2) and, we suggest that DuCV ORF2 and genomic distance genotype thresholds were 0.061 and 0.038, respectively.
已经使用标准化方法根据成对序列比较（PASC）来定义基因型。PASC 是病毒分类学中广泛接受的方法，它基于序列之间成对差异的直方图。最近，Zhang 等人（2013 年）得出结论，基因型 1 和 2 之间鸭圆环病毒（DuCV）的平均 p 距离为 0.170，DuCV-1 的亚型距离阈值为 0.032，DuCV-2 的亚型距离阈值为 0.018。然而，对于应用该方法来定义 DuCV 基因型可能存在一些担忧。考虑到上述担忧，我们的作者对包括 Zhang 等人（2013 年）的所有序列在内的 54 个衣壳基因（ORF2）和基因组序列进行了 PASC 分析。我们的结果证实了存在两种 DuCV 基因型（1 和 2），我们建议 DuCV ORF2 和基因组距离基因型阈值分别为 0.061 和 0.038。
