Geiger H H, Morgenstern K
Institut für Pflanzenzüchtung und Populationsgenetik und Landessaatzuchtanstalt, Universität Hohenheim, Stuttgart-Hohenheim, BRD.
Theor Appl Genet. 1975 Jan;46(6):269-76. doi: 10.1007/BF00281148.
Restorer test crosses of ten open-pollinated populations to CMS-line L1-P (P = 'Pampa' cytoplasm) in 1973 yielded 28 % male-fertile (mf), 27% partially male-sterile (pms), and 45 % male-sterile (ms) plants. In 1974, with CMS-tester-linie L3-P, the respective values were 7, 56 and 37 %. Significant differences between populations were found for the ms-proportions only. Averaged over the two tests they ranged from 16 to 57%.Tested to L1-P in 1971, 3 homozygous inbred lines were found to be restorers, 38 partial restorers, and 46 nonrestorers. Tested to L3-P in 1974, the respective numbers were 1,155 and 41 lines. 18 (out of 87) lines were classified in both tests as nonrestorers, but none as a restorer.Continued search for CMS sources was successful in 15 cases up to 1974. Four forms trace back to the Argentinian cultivar 'Pampa', ten to samples of Persian primitive rye and one to European adapted material.By means of diallel crosses, six genotypes (single crosses) were combined with three cytoplasms (Pampa-2 and two Persian sources). Three of the genotypes reacted equally with each of the cytoplasms: one as a restorer, one as a partial restorer, and one as a nonrestorer. The remaining combinations displayed pronounced plasmotype/ genotype interactions.Since 1971 an experiment has been in progress; to test the CMS mechanics for large scale hybrid seed production. Throughout, seed setting on the ms mother plants was similar to that on the pollinator plants. Fields were about 3 ha, and seed and pollinator parents were grown in strips 7, 50 m and 2, 50 m broad, respectively.
1973年，10个开放授粉群体与细胞质雄性不育系L1 - P（P = “潘帕”细胞质）进行恢复系测交，结果显示，雄性可育（mf）植株占28%，部分雄性不育（pms）植株占27%，雄性不育（ms）植株占45%。1974年，用细胞质雄性不育系测试系L3 - P进行测交，相应比例分别为7%、56%和37%。仅在雄性不育比例方面发现不同群体之间存在显著差异。两次测试的平均值在16%至57%之间。1971年对L1 - P进行测试时，发现3个纯合自交系为恢复系，38个为部分恢复系，46个为非恢复系。1974年对L3 - P进行测试时，相应数量分别为1个、155个和41个自交系。87个自交系中有18个在两次测试中均被归类为非恢复系，但没有一个是恢复系。到1974年为止，持续寻找细胞质雄性不育源的工作在15个案例中取得了成功。4种类型可追溯到阿根廷品种“潘帕”，10种类型可追溯到波斯原始黑麦样本，1种类型可追溯到欧洲适应材料。通过双列杂交，将6种基因型（单交种）与3种细胞质（潘帕 - 2和两种波斯来源）进行组合。其中3种基因型与每种细胞质的反应相同：一种为恢复系，一种为部分恢复系，一种为非恢复系。其余组合表现出明显的质型/基因型相互作用。自1971年以来一直在进行一项实验，以测试用于大规模杂交种子生产的细胞质雄性不育机制。总体而言，雄性不育母本植株的结实情况与授粉植株相似。试验田面积约为3公顷，种子亲本和授粉亲本分别种植成宽7.50米和2.50米的条带。
