Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, C.C.S., Bloco I, 21944-970, Ilha do Fundão, RJ, Brazil.
World J Microbiol Biotechnol. 1993 Sep;9(5):521-8. doi: 10.1007/BF00386287.
A bacterial strain, SCE2, identified as Bacillus polymyxa, produced an anti-microbial substance active against yeasts, fungi and different genera of Gram-positive and-negative bacteria, in liquid medium and in plate assays. This substance appeared to be an antibiotic different from the polymyxin group, mainly because of its action against the majority of Gram-positive bacteria tested and its lack of activity against Pseudomonas aeruginosa, a species usually killed by polymyxins. Preliminary characterization showed resistance to heat (65°C, 2 h), to proteases, trypsin, lysozyme, deoxyribonuclease I, ribonuclease A, phospholipase C, ethanol, acetone, chloroform, ether and to strong alkali treatment (2 M NaOH). The molecular weight was less than 3500. The B. polymyxa strain harboured a plasmid that did not correlate with antibiotic production; after curing experiments, a derivative strain, SCE2(46), was isolated that lacked the plasmid pES1, but showed the same inhibitory spectrum as the wild-type strain.
一种名为多粘类芽孢杆菌的细菌菌株 SCE2 在液体培养基和平板试验中产生了一种对酵母、真菌和不同属的革兰氏阳性和阴性细菌都有活性的抗菌物质。这种物质似乎是一种不同于多粘菌素类的抗生素，主要因为它对大多数测试的革兰氏阳性菌有作用，而对铜绿假单胞菌没有活性，而铜绿假单胞菌通常是多粘菌素类杀死的。初步特性表明，该物质对热（65°C，2 h）、蛋白酶、胰蛋白酶、溶菌酶、脱氧核糖核酸酶 I、核糖核酸酶 A、磷脂酶 C、乙醇、丙酮、氯仿、乙醚和强碱处理（2 M NaOH）有抗性。分子量小于 3500。多粘类芽孢杆菌菌株携带一种与抗生素产生无关的质粒；经过消除实验，分离出一个衍生菌株 SCE2(46)，该菌株缺乏质粒 pES1，但表现出与野生型菌株相同的抑制谱。
