Warren R L
Infect Immun. 1980 Nov;30(2):601-6. doi: 10.1128/iai.30.2.601-606.1980.
The plasmid contents of seven exfoliative toxin-producing strains of phage group 2 Staphylococcus aureus were analyzed by agarose gel electrophoresis and deoxyribonucleic acid-deoxyribonucleic acid hybridization. All strains were found to contain a large plasmid with a molecular weight of 27 X 10(6) except for strain RW1005. A comparison of the restriction endonuclease cleavage products by agarose gel electrophoresis showed that the number and size distribution of the fragments of all these Tox plasmids were similar, except for pRW002, which appeared to contain two deletions. Deoxyribonucleic acid-deoxyribonucleic acid hybridization studies confirmed that these plasmids were related to a plasmid which carried the genes for exfoliative toxin B and bacteriocin R1 biosynthesis and that they shared some sequence homology with the penicillinase plasmid pI258 isolated from a phage group 3 S. aureus.
采用琼脂糖凝胶电泳和脱氧核糖核酸-脱氧核糖核酸杂交技术，对噬菌体Ⅱ群金黄色葡萄球菌的7株产剥脱毒素菌株的质粒成分进行了分析。结果发现，除RW1005菌株外，所有菌株均含有一个分子量为27×10⁶的大质粒。通过琼脂糖凝胶电泳对限制性内切酶切割产物进行比较，结果显示，除pRW002似乎含有两个缺失片段外，所有这些产毒素质粒的片段数量和大小分布均相似。脱氧核糖核酸-脱氧核糖核酸杂交研究证实，这些质粒与携带剥脱毒素B基因和细菌素R1生物合成基因的质粒相关，并且它们与从噬菌体Ⅲ群金黄色葡萄球菌中分离出的青霉素酶质粒pI258具有一些序列同源性。
