海地地震前的非流行性霍乱弧菌
Pre-earthquake non-epidemic Vibrio cholerae in Haiti.
作者信息
Liu Jie, Winstead-Derlega Christopher, Houpt Eric, Heidkamp Rebecca, Pape Jean, Dillingham Rebecca
机构信息
University of Virginia, Charlottesville, VA, USA.
出版信息
J Infect Dev Ctries. 2014 Jan 15;8(1):120-2. doi: 10.3855/jidc.4524.
Abstract
INTRODUCTION
To our knowledge, there was no record of Vibrio cholerae in Haiti until the 2010 post earthquake outbreak.
METHODOLOGY
This study describes the analysis of 301 stool samples from 117 infants in Port-au-Prince, Haiti, who participated in a pediatric nutrition study between July 2008 and October 2009.
RESULTS
Nine samples were identified positive with both SYBR Green and Taqman-MGB probe based molecular assays targeting V. cholerae hlyA and toxR, respectively (Ct = 33-40), but none were O1 or O139.
CONCLUSIONS
Our results from multiple molecular assays demonstrate the presence of non-O1/O139 V. cholerae DNA in stools collected from nine asymptomatic Haitian infants two years prior to the 2010 earthquake.
摘要
引言
据我们所知，在2010年地震后霍乱爆发之前，海地没有霍乱弧菌的记录。
方法
本研究描述了对来自海地太子港117名婴儿的301份粪便样本的分析，这些婴儿在2008年7月至2009年10月期间参与了一项儿科营养研究。
结果
分别基于针对霍乱弧菌hlyA和toxR的SYBR Green和Taqman-MGB探针分子检测法，有9份样本被鉴定为阳性（Ct = 33 - 40），但均非O1或O139型。
结论
我们通过多种分子检测法得出的结果表明，在2010年地震前两年从九名无症状海地婴儿收集的粪便中存在非O1/O139型霍乱弧菌DNA。
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