Organic Chemistry Department, The Coca-Cola Company, Global Research and Development, One Coca-Cola Plaza, Atlanta, GA 30313, USA.
Int J Mol Sci. 2014 Jan 14;15(1):1014-25. doi: 10.3390/ijms15011014.
Degradation of rebaudioside M, a minor sweet component of Stevia rebaudiana Bertoni, under conditions that simulated extreme pH and temperature conditions has been studied. Thus, rebaudioside M was treated with 0.1 M phosphoric acid solution (pH 2.0) and 80 °C temperature for 24 h. Experimental results indicated that rebaudioside M under low pH and higher temperature yielded three minor degradation compounds, whose structural characterization was performed on the basis of 1D (1H-, 13C-) & 2D (COSY, HSQC, HMBC) NMR, HRMS, MS/MS spectral data as well as enzymatic and acid hydrolysis studies.
已研究了甜叶菊（Stevia rebaudiana Bertoni）中微量甜味成分甜菊双糖苷 M 在模拟极端 pH 值和温度条件下的降解情况。因此，用 0.1 M 磷酸溶液（pH 值 2.0）和 80°C 温度处理甜菊双糖苷 M 24 小时。实验结果表明，在低 pH 值和较高温度下，甜菊双糖苷 M 生成了三种微量降解化合物，并基于 1D（1H-、13C-）和 2D（COSY、HSQC、HMBC）NMR、高分辨质谱、质谱/质谱谱数据以及酶解和酸水解研究对其结构特征进行了表征。
