Toy Ebubekir, Malkoc Siddik, Corekci Bayram, Bozkurt Buket S, Hakki Sema S
Department of Orthodontics, Faculty of Dentistry, Inönü University, Malatya - Turkey.
J Appl Biomater Funct Mater. 2014 Dec 30;12(3):248-55. doi: 10.5301/JABFM.5000165.
The aim of this study was to evaluate the cytotoxic effects of 6 different orthodontic bracket types on human gingival fibroblasts (HGFs) using the xCELLigence system.
The orthodontic brackets used in this study were gold-plated steel (Apollo Gold), titanium (Rematitan), stainless steel (Equilibrium 2), lucid ice (Inspire ICE), metal-reinforced ceramic (Clarity) and composite (OrthoFlex). Gingival fibroblasts were isolated from human gingival connective tissue of systemically healthy individuals. Tested brackets were incubated in DMEM culture medium for 72 hours according to ISO 10993-5 standards. Gingival fibroblasts were maintained with Dulbecco modified Eagle medium containing 10% fetal bovine serum. The xCELLigence system was used to evaluate cell survival. The statistical analysis used was ANOVA and Tukey-Kramer multiple comparison tests.
When the data were evaluated in the 30th hour, Apollo Gold showed significant decreases in cell index (P<0.001). It also showed statistically significant decreases (P<0.001) in the 65th hour, but Clarity and Inspire ICE showed significant increases in cell indices (P<0.001, P<0.01). In the 114th hour, Clarity and Equilibrium 2 showed statistically significant increases in cell indices (P<0.001). Inspire ICE and Rematitan demonstrated significant increases (P<0.05). There were significant decreases in cell index of Apollo Gold (P<0.001).
The tested brackets are suitable for clinical application, but further studies using different test methods are needed for gold-plated brackets.
本研究旨在使用xCELLigence系统评估6种不同正畸托槽类型对人牙龈成纤维细胞（HGFs）的细胞毒性作用。
本研究中使用的正畸托槽为镀金钢托槽（Apollo Gold）、钛托槽（Rematitan）、不锈钢托槽（Equilibrium 2）、透明冰瓷托槽（Inspire ICE）、金属增强陶瓷托槽（Clarity）和复合托槽（OrthoFlex）。从全身健康个体的人牙龈结缔组织中分离出牙龈成纤维细胞。根据ISO 10993 - 5标准，将测试托槽在DMEM培养基中孵育72小时。牙龈成纤维细胞用含有10%胎牛血清的杜氏改良 Eagle培养基培养。使用xCELLigence系统评估细胞存活率。所采用的统计分析方法为方差分析和Tukey - Kramer多重比较检验。
在第30小时评估数据时，Apollo Gold托槽的细胞指数显著下降（P<0.001）。在第65小时也显示出统计学上的显著下降（P<0.001），但Clarity和Inspire ICE托槽的细胞指数显著增加（P<0.001，P<0.01）。在第114小时，Clarity和Equilibrium 2托槽的细胞指数显示出统计学上的显著增加（P<0.001）。Inspire ICE和Rematitan托槽显示出显著增加（P<0.05）。Apollo Gold托槽的细胞指数显著下降（P<0.001）。
测试的托槽适用于临床应用，但对于镀金托槽需要使用不同测试方法进行进一步研究。
