Escobar Lina M, Rivera Jaime Rodrigo, Arbelaez Eliana, Torres Luisa F, Villafañe Andrea, Díaz-Báez David, Mora Ingrid, Lafaurie Gloria I, Tanaka Midori
Unidad de Manejo Integral de Malformaciones Craneofaciales UMIMC, Universidad El Bosque, Bogotá, Colombia.
Posgrado de Ortodoncia, Facultad de Odontología, Universidad El Bosque, Bogotá, Colombia.
Int J Biomater. 2021 Jan 27;2021:8885290. doi: 10.1155/2021/8885290. eCollection 2021.
Orthodontic wires are made of alloys containing different metals, including nickel. It is important to evaluate their biocompatibility prior to use, owing to their long-term use in patients. This study compared the cytotoxicity and chemical composition of six latest orthodontic wires: Fantasia®, Tanzo®, FLI®, NT3®, DuoForce®, and Gummetal®. The before-use group consisted of wires that were not used in the mouth, and the after-use group consisted of wires that were used in the mouth for two months. The wires were placed in contact with human gingival fibroblasts (HGF) for 72 h, and cytotoxicity was determined using the resazurin test. The chemical composition and surface characterisation were evaluated by spectrometry and scanning electron microscopy. The groups were compared using ANOVA and Kruskal-Wallis test. Only the FLI® wires produced a 36% reduction in HGF viability ( < 0.05) and presented greater irregularities and loss of polymer structure. After-use wires showed a significant reduction in the percentage of nickel and the appearance of new elements (oxygen and carbon). Therefore, it can be concluded that no toxic ion release was noticed in this study. Rhodium-coated wires were more stable than PTFE-coated wires, and only the FLI® wires showed a slight cytotoxic effect.
正畸丝由包含不同金属（包括镍）的合金制成。由于它们在患者体内长期使用，在使用前评估其生物相容性很重要。本研究比较了六种最新正畸丝（Fantasia®、Tanzo®、FLI®、NT3®、DuoForce®和Gummetal®）的细胞毒性和化学成分。使用前组由未在口腔中使用的正畸丝组成，使用后组由在口腔中使用了两个月的正畸丝组成。将正畸丝与人类牙龈成纤维细胞（HGF）接触72小时，并使用刃天青试验测定细胞毒性。通过光谱分析和扫描电子显微镜评估化学成分和表面特征。使用方差分析和克鲁斯卡尔-沃利斯检验对各组进行比较。只有FLI®正畸丝使HGF活力降低了36%（P<0.05），且呈现出更大的不规则性和聚合物结构损失。使用后的正畸丝镍含量百分比显著降低，并出现了新元素（氧和碳）。因此，可以得出结论，本研究中未观察到有毒离子释放。镀铑正畸丝比聚四氟乙烯涂层正畸丝更稳定，只有FLI®正畸丝显示出轻微的细胞毒性作用。
