Department of Restorative Dentistry, Faculty of Dentistry, Inönü University, Malatya, Turkey.
Eur Rev Med Pharmacol Sci. 2013 Apr;17(7):984-8.
Achieving of moisture control especially gingival bleeding control is great challenge in clinical practice. Various hemostatic agents and techniques have been promoted for bleeding control during dental operation. But few studies have focused on the cytotoxicity of hemostatic solutions.
The aim of this study was to evaluate cytotoxic effect of hemostatic agents on human gingival fibroblast cells by using real-time cell analysis method.
Two hemostatic solutions, Hemoban (Sultan Healthcare, Hackensack, NJ, USA) and Hemasatic Solutions (W.P. Dental, Hamburg, Germany) that includes mainly aluminum chloride were used with different concentration. Gingival fibroblasts were isolated from gingival connective tissue during crown lengthening surgery of systemically healthy subjects. Gingival fibroblasts were maintained with Dulbecco's modified eagle medium containing 10% fetal bovine serum. A real-time cell analyzer (RT-CES, xCELLigence; Roche Applied Science, Mannheim, Germany, and ACEA Biosciences, San Diego, CA, USA) was used to evaluate cell survival. After seeding 200 mL of the cell suspensions into the wells (10,000 cells/well) of the E-plate 96, gingival fibroblasts were treated with hemostatic solutions (1/2, 1/4 and 1/8 dilutions) and monitored every 15 minutes for 72 hours. For the proliferation experiments, the statistical analyses used were 1-way analysis of variance (ANOVA) and Tukey HSD multiple comparisons tests.
According to statistically analysis, when evaluated at 48 and 72 hours, there were significant differences between the cell indexes of the control and all hemostatic agents groups (p < 0.001). Agent reduced cell index value significantly when compared to untreated control group.
The results indicate that using of Hemoban or Hemostatic Solutions as astringent solutions have a significant cytotoxic effect on gingival fibroblast cells.
在临床实践中，实现水分控制，尤其是牙龈出血控制是一个巨大的挑战。各种止血剂和技术已被推广用于控制牙科手术中的出血。但很少有研究关注止血溶液的细胞毒性。
本研究旨在通过实时细胞分析方法评估止血剂对人牙龈成纤维细胞的细胞毒性作用。
使用两种止血剂，Hemoban（Sultan Healthcare，Hackensack，NJ，USA）和Hemasatic Solutions（W.P. Dental，Hamburg，Germany），它们主要包含氯化铝，并用不同浓度使用。牙龈成纤维细胞是从系统健康受试者的冠延长手术中的牙龈结缔组织中分离出来的。牙龈成纤维细胞用含 10%胎牛血清的 Dulbecco 改良 Eagle 培养基维持。使用实时细胞分析仪（RT-CES，xCELLigence；Roche Applied Science，Mannheim，Germany 和 ACEA Biosciences，San Diego，CA，USA）评估细胞存活。将 200 mL 细胞悬浮液接种到 E-plate 96 的孔中（每孔 10,000 个细胞），用止血剂（1/2、1/4 和 1/8 稀释）处理牙龈成纤维细胞，并在 72 小时内每 15 分钟监测一次。对于增殖实验，使用的统计分析是单向方差分析（ANOVA）和 Tukey HSD 多重比较检验。
根据统计分析，在 48 小时和 72 小时时，对照组和所有止血剂组的细胞指数之间存在显著差异（p < 0.001）。与未处理的对照组相比，试剂显著降低了细胞指数值。
结果表明，Hemoban 或 Hemostatic Solutions 作为收敛剂溶液对牙龈成纤维细胞具有显著的细胞毒性作用。
