State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
Int J Syst Evol Microbiol. 2014 Mar;64(Pt 3):963-967. doi: 10.1099/ijs.0.054015-0. Epub 2013 Dec 18.
A taxonomic study was carried out on strain GCS-AN-3(T), which was isolated from a phenol-degrading consortium enriched from coking wastewater activated sludge of Beijing Shougang Company Limited during the screening of phenol-degrading bacteria. Cells of strain GCS-AN-3(T) were Gram-stain-negative, short rods, and oxidase-/catalase-positive. Growth was observed at salinities from 0 to 2.5 % and at temperatures from 10 to 37 °C. 16S rRNA gene sequence analysis showed that strain GCS-AN-3(T) was most closely related to Ottowia pentelensis DSM 21699(T) (96.2 %). The principal fatty acids were summed feature 3 (C16 : 1ω7c/C16 : 1ω6c), C16 : 0, summed feature 8 (C18 : 1ω7c/C18 : 1ω6c) and cyclo C17 : 0. The major respiratory quinone was Q-8. The polar lipids comprised phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. The G+C content of the genomic DNA was 67.6 mol%. Thiosulfate could be utilized as co-substrate for aerobic growth and was oxidized to sulfate. On the basis of phenotypic, chemotaxonomic and molecular data, strain GCS-AN-3(T) is considered to represent a novel species of the genus Ottowia, for which the name Ottowia beijingensis sp. nov. is proposed (type strain GCS-AN-3(T) = LMG 27179(T) = CGMCC 1.12324(T) = MCCC 1A01410(T)). An emended description of the genus Ottowia is also proposed.
一项关于菌株 GCS-AN-3(T)的分类学研究，该菌株是从北京首钢有限公司焦化废水活性污泥中富集的苯酚降解菌中筛选出的。菌株 GCS-AN-3(T)的细胞革兰氏阴性，短杆状，氧化酶+/过氧化氢酶阳性。在盐度为 0 至 2.5%和温度为 10 至 37°C 的条件下均可生长。16S rRNA 基因序列分析表明，菌株 GCS-AN-3(T)与 Ottowia pentelensis DSM 21699(T)（96.2%）最为密切相关。主要脂肪酸为(summed feature 3，C16:1ω7c/C16:1ω6c)、C16:0、(summed feature 8，C18:1ω7c/C18:1ω6c)和环 C17:0。主要呼吸醌为 Q-8。极性脂包括磷脂酰胆碱、磷脂酰甘油、磷脂酰乙醇胺和一种未知的磷脂。基因组 DNA 的 G+C 含量为 67.6 mol%。硫代硫酸盐可作为好氧生长的共底物，并被氧化为硫酸盐。基于表型、化学分类学和分子数据，菌株 GCS-AN-3(T)被认为代表 Ottowia 属的一个新种，建议将其命名为 Ottowia beijingensis sp. nov.（模式菌株 GCS-AN-3(T) = LMG 27179(T) = CGMCC 1.12324(T) = MCCC 1A01410(T)）。还提出了 Ottowia 属的修订描述。
