Marie College, Shandong University, Weihai, Shandong, 264209, PR China.
Department of Central Lab, Weihai Municipal Hospital. Weihai, Shandong, 264200, PR China.
Int J Syst Evol Microbiol. 2024 May;74(5). doi: 10.1099/ijsem.0.006360.
A Gram-negative, facultative anaerobic, non-motile and rod-shaped bacterium, designated 10c7w1, was isolated from a human gastrointestinal tract. Colonies on agar plates were small, circular, smooth and beige. The optimal growth conditions were determined to be 37 °C, pH 7.0-7.5 and 0 % (w/v) NaCl. Comparative analysis of complete 16S rRNA gene sequences revealed that strain 10c7w1 showed the highest sequence similarity of 95.8 % to MCCC 1A01410, followed by (95.2 %) JCM 11629. The average amino acid identity values between 10c7w1 and MCCC 1A01410 and JCM 11629 were above 60 % (71.4 and 69.5 %). The average nucleotide identity values between strain 10c7w1 and MCCC 1A01410 and JCM 11629 were 76.9 and 72.5 %, respectively. The dominant fatty acids (≥10 %) were straight chain ones, with summed feature 3 (C 7/C 6), summed feature 8 (C 7/C 6) and C being the most abundant. Q-8 was the only respiratory quinone. The major polar lipids of strain 10c7w1 were phosphatidylethanolamine, diphosphatidylglycerol and unknown lipids. The DNA G+C content of strain 10c7w1 was 63.6 mol%. On the basis of phylogenetic, phenotypic and chemotaxonomic data, strain 10c7w1 is considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 10c7w1 (=MCCC 1H01399=KCTC 92200).
一株革兰氏阴性、兼性厌氧、无动力、杆状细菌，被命名为 10c7w1，从人体胃肠道中分离得到。琼脂平板上的菌落小而圆，光滑，呈米色。最佳生长条件确定为 37°C、pH7.0-7.5 和 0%（w/v）NaCl。对完整 16S rRNA 基因序列的比较分析表明，菌株 10c7w1 与 MCCC 1A01410 的序列相似度最高，为 95.8%，其次是 JCM 11629，为 95.2%。10c7w1 与 MCCC 1A01410 和 JCM 11629 的平均氨基酸同一性值均在 60%以上（分别为 71.4%和 69.5%）。菌株 10c7w1 与 MCCC 1A01410 和 JCM 11629 的平均核苷酸同一性值分别为 76.9%和 72.5%。菌株 10c7w1 的优势脂肪酸（≥10%）为直链脂肪酸，特征 3（C7/C6）、特征 8（C7/C6）和 C 总和最高。Q-8 是唯一的呼吸醌。菌株 10c7w1 的主要极性脂为磷脂酰乙醇胺、双磷脂酰甘油和未知脂。菌株 10c7w1 的 DNA G+C 含量为 63.6mol%。基于系统发育、表型和化学分类学数据，菌株 10c7w1 被认为代表一个新的属种，命名为 sp. nov.。模式菌株为 10c7w1（=MCCC 1H01399=KCTC 92200）。
