从口腔病原体弯曲菌中鉴定和描述入侵抗原 B 基因。
Identification and Characterization of an Invasion Antigen B Gene from the Oral Pathogen Campylobacter rectus.
机构信息
Department of Biological Sciences, Florida Gulf Coast University, 10501 FGCU Boulevard South, Fort Myers, FL 33965 USA.
Department of Microbiology, North Carolina State University, Raleigh, NC USA.
出版信息
Indian J Microbiol. 2014 Mar;54(1):33-40. doi: 10.1007/s12088-013-0406-z. Epub 2013 Apr 21.
The oral bacterium, Campylobacter rectus, is an etiological agent of periodontitis. The virulence genes of C. rectus are largely unknown. The aim of this study was to query C. rectus for the presence of an invasion antigen B (ciaB) gene, which is needed for cell invasion by the related species Campylobacter jejuni. PCR and PCR-walking identified a ciaB from C. rectus. In silico analyses of C. rectus 314 ciaB (Cr-ciaB) revealed an ORF of 1,830 base pairs. The Cr-CiaB protein shared significant sequence identity (BLASTx and phylogeny) with CiaB from related campylobacters. Cr-CiaB is predicted to lack membrane helices, signal peptides, and localizes to the cytoplasm; which are consistent with CiaB proteins. Expression of Cr-ciaB was confirmed with RT-PCR; and potential ciaB genes were detected in eight additional strains of C. rectus. Cr-ciaB is the first CiaB identified from the oral campylobacters.
口腔细菌直肠弯曲杆菌是牙周炎的病因之一。直肠弯曲杆菌的毒力基因在很大程度上是未知的。本研究旨在查询直肠弯曲杆菌中是否存在侵袭抗原 B（ciaB）基因，该基因是相关物种空肠弯曲杆菌细胞侵袭所必需的。PCR 和 PCR 漫步鉴定出来自直肠弯曲杆菌的 ciaB。对直肠弯曲杆菌 314 个 ciaB（Cr-ciaB）的计算机分析显示，其 ORF 为 1830 个碱基对。Cr-CiaB 蛋白与相关弯曲杆菌的 CiaB 具有显著的序列同一性（BLASTx 和系统发育）。Cr-CiaB 预测缺乏膜螺旋、信号肽，并定位于细胞质；这与 CiaB 蛋白一致。用 RT-PCR 证实了 Cr-ciaB 的表达；并在另外 8 株直肠弯曲杆菌中检测到潜在的 ciaB 基因。Cr-ciaB 是从口腔弯曲杆菌中鉴定出的第一个 CiaB。
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